Cas9 deactivation with photocleavable guide RNAs

Roger S. Zou, Yang Liu, Bin Wu, Taekjip Ha

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.

Original languageEnglish (US)
Pages (from-to)1553-1565.e8
JournalMolecular cell
Issue number7
StatePublished - Apr 1 2021


  • Cas9
  • DNA repair
  • deactivation
  • genome editing
  • specificity

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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