@article{ecc43876b07e443aa6177292f2bae015,
title = "Cas9 deactivation with photocleavable guide RNAs",
abstract = "Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.",
keywords = "CRISPR, Cas9, DNA repair, deactivation, genome editing, specificity",
author = "Zou, {Roger S.} and Yang Liu and Bin Wu and Taekjip Ha",
note = "Funding Information: We thank Dr. Shiva Razavi and Shuaixin He for assistance with protein purification, Dr. Geraldine Seydoux for access to the Lonza 4D nucleofector, Dr. Winston Timp for access to a MiSeq and Bioruptor Pico sonicator, and Dr. Haiping Hao of the Transcriptomics and Deep Sequencing Core for sequencing of ChIP samples. This work was supported by grants from the NIH ( R35 GM 122569 and U01 DK 127432 to T.H. and T32 GM 73009 and F30 CA 254160 to R.S.Z.), National Science Foundation (NSF) ( PHY 1430124 and EFMA 1933303 to T.H., 1817447 to B.W.) and Pew Charitable Trust ( 00030601 to B.W.). T.H. is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = apr,
day = "1",
doi = "10.1016/j.molcel.2021.02.007",
language = "English (US)",
volume = "81",
pages = "1553--1565.e8",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "7",
}