TY - JOUR
T1 - Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status
AU - Afzal, Junaid
AU - Chan, Angel
AU - Karakas, Mehmet Fatih
AU - Woldemichael, Kirubel
AU - Vakrou, Styliani
AU - Guan, Yufan
AU - Rathmell, Jeffrey
AU - Wahl, Richard
AU - Pomper, Martin
AU - Foster, D. Brian
AU - Aon, Miguel A.
AU - Tsui, Benjamin
AU - O'Rourke, Brian
AU - Abraham, M. Roselle
N1 - Funding Information:
This work was funded by the American Heart Association (AHA-BGIA and AHA-GIA) and the National Institutes of Health (NIH) (RO1 HL092985 and UL1 RR 025005). Dr. Chan was supported by NIH Training Grant NIHT32HL07227. Dr. Karakas was supported by a TUBITAK2219 Research Programme Grant and Fulbright Grant. Dr. Woldemichael was supported by NIH Diversity Fellowships (R01HL092985). Dr. Vakrou was supported by a fellowship from the Hellenic Heart Failure Society. Dr. Tsui has received a royalty from GE Healthcare; and has received a research contract from Philips Healthcare. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose. Drs. Afzal and Chan contributed equally to this work. Robert Roberts, MD, served as Guest Editor for this paper.
Publisher Copyright:
© 2017 The Authors
PY - 2017/10
Y1 - 2017/10
N2 - Adult stem cells demonstrate metabolic flexibility that is regulated by cell adhesion status. The authors demonstrate that adherent cells primarily utilize glycolysis, whereas suspended cells rely on oxidative phosphorylation for their ATP needs. Akt phosphorylation transduces adhesion-mediated regulation of energy metabolism, by regulating translocation of glucose transporters (GLUT1) to the cell membrane and thus, cellular glucose uptake and glycolysis. Cell dissociation, a pre-requisite for cell transplantation, leads to energetic stress, which is mediated by Akt dephosphorylation, downregulation of glucose uptake, and glycolysis. They designed hydrogels that promote rapid cell adhesion of encapsulated cells, Akt phosphorylation, restore glycolysis, and cellular ATP levels.
AB - Adult stem cells demonstrate metabolic flexibility that is regulated by cell adhesion status. The authors demonstrate that adherent cells primarily utilize glycolysis, whereas suspended cells rely on oxidative phosphorylation for their ATP needs. Akt phosphorylation transduces adhesion-mediated regulation of energy metabolism, by regulating translocation of glucose transporters (GLUT1) to the cell membrane and thus, cellular glucose uptake and glycolysis. Cell dissociation, a pre-requisite for cell transplantation, leads to energetic stress, which is mediated by Akt dephosphorylation, downregulation of glucose uptake, and glycolysis. They designed hydrogels that promote rapid cell adhesion of encapsulated cells, Akt phosphorylation, restore glycolysis, and cellular ATP levels.
KW - SPECT imaging
KW - cardiosphere-derived cells (CDCs)
KW - glycolysis
KW - metabolism
KW - oxidative phosphorylation (OxPhos)
KW - sodium-iodide symporter (NIS)
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U2 - 10.1016/j.jacbts.2017.03.016
DO - 10.1016/j.jacbts.2017.03.016
M3 - Article
C2 - 29520378
AN - SCOPUS:85032708357
SN - 2452-302X
VL - 2
SP - 543
EP - 560
JO - JACC: Basic to Translational Science
JF - JACC: Basic to Translational Science
IS - 5
ER -