TY - JOUR
T1 - Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents
AU - Zhang, Jia
AU - Yuan, Yue
AU - Gao, Minling
AU - Han, Zheng
AU - Chu, Chengyan
AU - Li, Yuguo
AU - van Zijl, Peter C.M.
AU - Ying, Mingyao
AU - Bulte, Jeff W.M.
AU - Liu, Guanshu
N1 - Funding Information:
Financial support from the NIH grants R03 EB021573, R01 CA211087, R21 CA215860, R01 EB015032, R01 EB023647, R01 DK106972, R56 NS098520, and P41 EB024495 is acknowledged. We thank Dr. Patricia McGuiggan for gen- erous help in performing AFM, Dr. Joel Tang for assistance in conducting FTIR, Dr. Katherine Tripp for help with the in vitro fluorescence measurements, Dr. Scott Kuo for invaluable suggestions, and Dr. Jiadi Xu for help with the mouse MRI experiment.
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/7/15
Y1 - 2019/7/15
N2 - While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.
AB - While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.
KW - CEST MRI
KW - carbon dots
KW - cell labeling
KW - contrast agent
KW - intracranial implantation
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U2 - 10.1002/anie.201904722
DO - 10.1002/anie.201904722
M3 - Article
C2 - 31162873
AN - SCOPUS:85068064240
SN - 1433-7851
VL - 58
SP - 9871
EP - 9875
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 29
ER -