cAMP Stimulates SLC26A3 Activity in Human Colon by a CFTR-Dependent Mechanism That Does Not Require CFTR Activity

Chung Ming Tse, Jianyi Yin, Varsha Singh, Rafiquel Sarker, Ruxian Lin, Alan S. Verkman, Jerrold R. Turner, Mark Donowitz

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Background & Aims: SLC26A3 (DRA) is an electroneutral Cl - /HCO 3 - exchanger that is present in the apical domain of multiple intestinal segments. An area that has continued to be poorly understood is related to DRA regulation in acute adenosine 3′,5′-cyclic monophosphate (cAMP)-related diarrheas, in which DRA appears to be both inhibited as part of NaCl absorption and stimulated to contribute to increased HCO 3 - secretion. Different cell models expressing DRA have shown that cAMP inhibits, stimulates, or does not affect its activity. Methods: This study re-evaluated cAMP regulation of DRA using new tools, including a successful knockout cell model, a specific DRA inhibitor (DRA inh -A250), specific antibodies, and a transport assay that did not rely on nonspecific inhibitors. The studies compared DRA regulation in colonoids made from normal human colon with regulation in the colon cancer cell line, Caco-2. Results: DRA is an apical protein in human proximal colon, differentiated colonoid monolayers, and Caco-2 cells. It is glycosylated and appears as 2 bands. cAMP (forskolin) acutely stimulated DRA activity in human colonoids and Caco-2 cells. In these cells, DRA is the predominant apical Cl - /HCO 3 - exchanger and is inhibited by DRA inh -A250 with a median inhibitory concentration of 0.5 and 0.2 μmol/L, respectively. However, there was no effect of cAMP in HEK293/DRA cells that lacked a cystic fibrosis transmembrane conductance regulator (CFTR). When CFTR was expressed in HEK293/DRA cells, cAMP also stimulated DRA activity. In all cases, cAMP stimulation of DRA was not inhibited by CFTR inh -172. Conclusions: DRA is acutely stimulated by cAMP by a process that is CFTR-dependent, but appears to be one of multiple regulatory effects of CFTR that does not require CFTR activity.

Original languageEnglish (US)
Pages (from-to)641-653
Number of pages13
Issue number3
StatePublished - Jan 1 2019


  • CFTR
  • Cl /HCO Exchange
  • Colon
  • Enteroids
  • Secretory Diarrhea

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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