Calmodulin kinase determines calcium-dependent facilitation of L-type calcium channels

Igor Dzhura, Yuejin Wu, Roger J. Colbran, Jeffrey R. Balser, Mark E. Anderson

Research output: Contribution to journalArticlepeer-review

297 Scopus citations


A dynamic positive feedback mechanism, known as 'facilitation', augments L-type calcium-ion currents (I(Ca)) in response to increased intracellular Ca2+ concentrations. The Ca2+-binding protein calmodulin (CAM) has been implicated in facilitation, but the single-channel signature and the signalling events underlying Ca2+/CaM-dependent facilitation are unknown. Here we show that the Ca2+/CaM-dependent protein kinase II (CaMK) is necessary and possibly sufficient for I(Ca) facilitation. CaMK induces a channel-gating mode that is characterized by frequent, long openings of L-type Ca2+ channels. We conclude that CaMK-mediated phosphorylation is an essential signalling event in triggering Ca2+/CaM-dependent I(Ca) facilitation.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalNature cell biology
Issue number3
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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