Calibration of an eye oximeter with a dynamic eye phantom

A. Nabili, D. Bardakci, K. Helling, C. Matyas, S. Muro, J. C. Ramella-Roman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Measurements of oxygen saturation and flow in the retina can yield information about the eye health and the onset of eye pathologies such as Diabetic Retinopathy. Recently we have realized an instrument capable of measuring oxygenation in the retina using six different wavelengths and capable of measuring blood flow using speckle-based techniques. The calibration of such instrument is particularly difficult due to the layered structure of the eye and the lack of alternative measurement techniques. For this purpose we have realized an in vitro model of the human eye. The artificial eye is composed of four layers: the retina vessels, the choroids, the retinal pigmented epithelium (RPE), and the sclera. The retina vessels are modeled with 150 μm tube connected to a micro-pump delivering 34 μl/min. The micro-tube, the pump, and a blood reservoir were connected in a closed circulatory system; blood oxygenation in the vessel could be modified using an external oxygen reservoir. The optical properties of all other layers were mimicked using titanium dioxide as a scatterer and ink as an absorber. The absorption coefficient μa and the scattering coefficient μs of these layers were independently measured using an integrating sphere. Absorption and scattering coefficient of all layers were modified before experimental measurements and a Monte Carlo program was finally used to model the experimental results.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2008
Externally publishedYes
EventDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue - San Jose, CA, United States
Duration: Jan 19 2008Jan 21 2008


OtherDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
Country/TerritoryUnited States
CitySan Jose, CA


  • Eye oximetry
  • Optical phantoms

ASJC Scopus subject areas

  • Engineering(all)


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