Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells

Haiqiang Dou, Changhe Wang, Xi Wu, Lijun Yao, Xiaoyu Zhang, Sasa Teng, Huadong Xu, Bin Liu, Qihui Wu, Quanfeng Zhang, Meiqin Hu, Yeshi Wang, Li Wang, Yi Wu, Shujiang Shang, Xinjiang Kang, Lianghong Zheng, Jin Zhang, Matthieu Raoux, Jochen LangQing Li, Jing Su, Xiao Yu, Liangyi Chen, Zhuan Zhou

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Aims/hypothesis: Insulin is a key metabolic regulator in health and diabetes. In pancreatic beta cells, insulin release is regulated by the major second messengers Ca2+ and cAMP: exocytosis is triggered by Ca2+ and mediated by the cAMP/protein kinase A (PKA) signalling pathway. However, the causal link between these two processes in primary beta cells remains undefined.

Results: Membrane depolarisation-induced Ca2+ influx caused an increase in cytosolic PKA activity via activating a Ca2+-sensitive adenylyl cyclase 8 (ADCY8) subpool. Glucose stimulation triggered coupled Ca2+ oscillations and PKA activation. ADCY8 knockdown significantly reduced the level of depolarisation-evoked PKA activation and impaired replenishment of the readily releasable vesicle pool. Pharmacological inhibition of PKA by two inhibitors reduced depolarisation-induced PKA activation to a similar extent and reduced the capacity for sustained vesicle exocytosis and insulin release.

Conclusions/interpretation: Our findings suggest that depolarisation-induced Ca2+ influx plays dual roles in regulating exocytosis in rat pancreatic beta cells by triggering vesicle fusion and replenishing the vesicle pool to support sustained insulin release. Therefore, Ca2+ influx may be important for glucose-stimulated insulin secretion.

Methods: Time-resolved confocal imaging of fluorescence resonance energy transfer signals was performed to visualise PKA activity, and combined membrane capacitance recordings were used to monitor insulin secretion from patch-clamped rat beta cells.

Original languageEnglish (US)
Pages (from-to)324-333
Number of pages10
Issue number2
StatePublished - 2014


  • Adenylyl cyclase 8
  • Ca<sup>2+</sup>
  • Pancreatic beta cell
  • Protein kinase A
  • Vesicle pool

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • General Medicine


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