Broad identification of bacterial type in urinary tract infection using 1H NMR spectroscopy

Ashish Gupta, Mayank Dwivedi, Abbas Ali Mahdi, Chunni Lal Khetrapal, Mahendra Bhandari

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

To address the shortcomings of urine culture for the rapid identification of urinary tract infection (UTI), we applied 1H-nuclear magnetic resonance (NMR) spectroscopy as a surrogate method for fast screening of microorganisms. Study includes 682 urine samples from suspected UTI patients, 50 healthy volunteers, and commercially available standard strains of gram negative bacilli (GNB) (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacter, Acinetobacter, Proteus mirabilis, Citrobacter frundii) and gram positive cocci (GPC) (Enterococcus faecalis, Streptococcus group B, Staphylococcus saprophyticus). Acetate, lactate, ethanol, succinate, creatinine, trimethylamine (TMA), citrate, trimethylamin-N-oxide, glycine, urea, and hippurate were measured by 1H NMR spectroscopy. All urine specimens were evaluated with culture method. Multivariate discriminant function analysis (DFA) reveals that acetate, lactate, succinate, and formate were able to differentiate, with high accuracy (99.5%), healthy controls from UTI patients. This statistical analysis was also able to classify GNB to GPC infected urine samples with high accuracy (96%). This technique appears to be a promising, rapid, and noninvasive approach to probing GNB and GPC infected urine specimens with its distinguishing metabolic profile. The determination of infection will be very important for rapidly and efficiently measuring the efficacy of a tailored treatment, leading to prompt and appropriate care of UTI patients.

Original languageEnglish (US)
Pages (from-to)1844-1854
Number of pages11
JournalJournal of proteome research
Volume11
Issue number3
DOIs
StatePublished - Mar 2 2012

Keywords

  • NMR spectroscopy
  • UTI
  • urine metabolic profile

ASJC Scopus subject areas

  • Biochemistry
  • General Chemistry

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