TY - JOUR
T1 - Blue eye disease porcine rubulavirus (PoRv) infects pig neurons and glial cells using sialo-glycoprotein as receptor
AU - Mendoza-Magaña, Maria L.
AU - Godoy-Martinez, Diana V.
AU - Guerrero-Cazares, Hugo
AU - Rodriguez-Peredo, Alejandra
AU - Dueñas-Jimenez, Judith M.
AU - Dueñas-Jiménez, Sergio H.
AU - Ramírez-Herrera, Mario A.
N1 - Funding Information:
This work was supported by: Consejo Nacional de Ciencia y Tecnologia (CONACYT): 38219-N.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/3
Y1 - 2007/3
N2 - Pig neural cells express glycoproteins with sialylated N-linked oligosaccharide chains (SNOC) which are used by the porcine rubulavirus (PoRv) as receptors. Pig neuronal or glial cell cultures were employed to investigate (a) whether PoRv infects such cells using a molecule expressing SNOC, and (b) the role of viral envelope glycoproteins in establishing the infection. Enriched neuronal or glial cell cultures were exposed to PoRv and infection was detected immunocytochemically. Neuronal cultures prepared from neonatal pigs were treated enzymatically to eliminate sialic acid or N-linked oligosaccharide chains. Primary neural cultures were exposed to anti-HN or anti-F preincubated with PoRv to study the role of the viral glycoproteins. In enriched cultures, PoRv infected neurons and glial cells, and sialic acid expressed in N-linked oligosaccharide chains appeared to play a central role in infection. It was concluded that HN and F viral glycoproteins are required to infect neurons and glial cells.
AB - Pig neural cells express glycoproteins with sialylated N-linked oligosaccharide chains (SNOC) which are used by the porcine rubulavirus (PoRv) as receptors. Pig neuronal or glial cell cultures were employed to investigate (a) whether PoRv infects such cells using a molecule expressing SNOC, and (b) the role of viral envelope glycoproteins in establishing the infection. Enriched neuronal or glial cell cultures were exposed to PoRv and infection was detected immunocytochemically. Neuronal cultures prepared from neonatal pigs were treated enzymatically to eliminate sialic acid or N-linked oligosaccharide chains. Primary neural cultures were exposed to anti-HN or anti-F preincubated with PoRv to study the role of the viral glycoproteins. In enriched cultures, PoRv infected neurons and glial cells, and sialic acid expressed in N-linked oligosaccharide chains appeared to play a central role in infection. It was concluded that HN and F viral glycoproteins are required to infect neurons and glial cells.
KW - Cell receptor
KW - Glycosidase
KW - Neuronal culture
KW - Rubulavirus
KW - Viral infection
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U2 - 10.1016/j.tvjl.2005.12.004
DO - 10.1016/j.tvjl.2005.12.004
M3 - Article
C2 - 16459111
AN - SCOPUS:33847040728
SN - 1090-0233
VL - 173
SP - 428
EP - 436
JO - British Veterinary Journal
JF - British Veterinary Journal
IS - 2
ER -