Blood–Brain Glucose Transport in the Conscious Rat: Comparison of the Intravenous and Intracarotid Injection Methods

Albert Gjedde; Mette Rasmussen

doi:10.1111/j.1471-4159.1980.tb09012.x

Blood–Brain Glucose Transport in the Conscious Rat: Comparison of the Intravenous and Intracarotid Injection Methods

Albert Gjedde, Mette Rasmussen

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Abstract: The unidirectional transfer of d‐glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood–brain glucose transfer rate (T_max) was 407 μ mol (100 g)^–1 min^–1 when measured by intravenous injection, and 352 μ mol (100 g)^–1 min^–1 when measured by intracarotid injection. The half–saturation constants (K_m) were 7.8 mm and 16.8 HIM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.

Original language	English (US)
Pages (from-to)	1375-1381
Number of pages	7
Journal	Journal of Neurochemistry
Volume	35
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-4159.1980.tb09012.x
State	Published - Dec 1980
Externally published	Yes

Keywords

Blood‐brain barrier
Blood‐brain glucose transfer

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1980.tb09012.x

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abstract = "Abstract: The unidirectional transfer of d‐glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood–brain glucose transfer rate (Tmax) was 407 μ mol (100 g)–1 min–1 when measured by intravenous injection, and 352 μ mol (100 g)–1 min–1 when measured by intracarotid injection. The half–saturation constants (Km) were 7.8 mm and 16.8 HIM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.",

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AU - Rasmussen, Mette

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N2 - Abstract: The unidirectional transfer of d‐glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood–brain glucose transfer rate (Tmax) was 407 μ mol (100 g)–1 min–1 when measured by intravenous injection, and 352 μ mol (100 g)–1 min–1 when measured by intracarotid injection. The half–saturation constants (Km) were 7.8 mm and 16.8 HIM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.

AB - Abstract: The unidirectional transfer of d‐glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood–brain glucose transfer rate (Tmax) was 407 μ mol (100 g)–1 min–1 when measured by intravenous injection, and 352 μ mol (100 g)–1 min–1 when measured by intracarotid injection. The half–saturation constants (Km) were 7.8 mm and 16.8 HIM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.

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KW - Blood‐brain glucose transfer

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Blood–Brain Glucose Transport in the Conscious Rat: Comparison of the Intravenous and Intracarotid Injection Methods

Abstract

Keywords

ASJC Scopus subject areas

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