@inbook{c6120bcce68a408d92f293b104f79005,
title = "Bioreducible poly(Beta-amino ester)s for intracellular delivery of SiRNA",
abstract = "RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequencespecific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications.",
keywords = "Gene delivery, Nanoparticle, Polymer, RNA interference, SiRNA, Transfection",
author = "Kozielski, {Kristen L.} and Green, {Jordan J.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3112-5_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
booktitle = "Methods in Molecular Biology",
}