Batch Rearing Aedes aegypti

Margot P. Wohl; Conor J. McMeniman

doi:10.1101/pdb.prot108017

Batch Rearing Aedes aegypti

Margot P. Wohl, Conor J. McMeniman

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Standardized rearing methods for the yellow fever mosquito Aedes aegypti are critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol for Aedes aegypti stocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feeding Aedes aegypti to yield viable eggs for continuous culture or storage, are detailed.

Original language	English (US)
Pages (from-to)	172-181
Number of pages	10
Journal	Cold Spring Harbor Protocols
Volume	2023
Issue number	3
DOIs	https://doi.org/10.1101/pdb.prot108017
State	Published - Mar 2023

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot108017

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abstract = "Standardized rearing methods for the yellow fever mosquito Aedes aegypti are critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol for Aedes aegypti stocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feeding Aedes aegypti to yield viable eggs for continuous culture or storage, are detailed.",

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note = "Funding Information: preceded us. We thank the National Institutes of Health (R21 AI146450-01), Innovative Vector Control Consortium, Johns Hopkins Malaria Research Institute, and Bloomberg Philanthropies for generous funding to C.J.M. and a postdoctoral fellowship to M.P.W. from The Molecular and Cellular Basis of Infectious Diseases (MCBID) Program (T32A1007417) funded by the National Institutes of Health. Publisher Copyright: {\textcopyright} 2023 Cold Spring Harbor Laboratory Press.",

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Batch Rearing Aedes aegypti

Abstract

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