Banded structures in collagen vitrigels for corneal injury repair

Zhiyong Xia; Xiomara Calderón-Colón; Russell McCally; Jeffrey Maranchi; Lixia Rong; Benjamin Hsiao; Jennifer Elisseeff; Morgana Trexler

doi:10.1016/j.actbio.2014.05.010

Banded structures in collagen vitrigels for corneal injury repair

Zhiyong Xia, Xiomara Calderón-Colón, Russell McCally, Jeffrey Maranchi, Lixia Rong, Benjamin Hsiao, Jennifer Elisseeff, Morgana Trexler

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64 nm (by SAXS) or 57 nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33 nm and from 23 to 25 nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.

Original language	English (US)
Pages (from-to)	3615-3619
Number of pages	5
Journal	Acta Biomaterialia
Volume	10
Issue number	8
DOIs	https://doi.org/10.1016/j.actbio.2014.05.010
State	Published - Aug 2014

Keywords

Banded structure
Corneal injury repair
D-Spacing
Small-angle X-ray scattering
collagen vitrigel

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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10.1016/j.actbio.2014.05.010

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title = "Banded structures in collagen vitrigels for corneal injury repair",

abstract = "There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64 nm (by SAXS) or 57 nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33 nm and from 23 to 25 nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.",

keywords = "Banded structure, Corneal injury repair, D-Spacing, Small-angle X-ray scattering, collagen vitrigel",

author = "Zhiyong Xia and Xiomara Calder{\'o}n-Col{\'o}n and Russell McCally and Jeffrey Maranchi and Lixia Rong and Benjamin Hsiao and Jennifer Elisseeff and Morgana Trexler",

note = "Funding Information: This research was sponsored by the US Department of the Army under award number W81XWH-09-2-0173 . The U.S. Army Medical Research Acquisition Activity , 820 Chandler Street, Fort Detrick, MD 21702-5014 is the awarding and administering acquisition office. The content of this article does not necessarily reflect the position or the policy of the government, and no official endorsement should be inferred. ",

year = "2014",

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doi = "10.1016/j.actbio.2014.05.010",

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AU - Xia, Zhiyong

AU - Calderón-Colón, Xiomara

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AU - Rong, Lixia

AU - Hsiao, Benjamin

AU - Elisseeff, Jennifer

AU - Trexler, Morgana

N1 - Funding Information: This research was sponsored by the US Department of the Army under award number W81XWH-09-2-0173 . The U.S. Army Medical Research Acquisition Activity , 820 Chandler Street, Fort Detrick, MD 21702-5014 is the awarding and administering acquisition office. The content of this article does not necessarily reflect the position or the policy of the government, and no official endorsement should be inferred.

PY - 2014/8

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Banded structures in collagen vitrigels for corneal injury repair

Abstract

Keywords

ASJC Scopus subject areas

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