Bacteroides fragilis enterotoxin induces c-Myc expression and cellular proliferation

Background & Aims: Enterotoxigenic Bacteroides fragilis that secrete a zinc-dependent metalloprotease toxin termed the B. fragilis toxin (BFT) have been associated with acute diarrheal disease. BFT rapidly cleaves the extracellular domain of E-cadherin, leading to the complete degradation of the E-cadherin protein. E-cadherin is the primary intercellular adhesion protein of the zonula adherens, and its cytoplasmic domain associates with the nuclear signaling protein β-catenin. The goal of this study was to examine if BFT triggers β-catenin nuclear signaling in intestinal epithelial cells. Methods: Cell biologic and biochemical techniques were combined to address β-catenin nuclear signaling stimulated by BFT. Results: Loss of membrane-associated E-cadherin after BFT treatment of human colonic epithelial cells (HT29/C1 clone) triggers β-catenin nuclear localization within 3 hours. Subsequently, c-myc transcription and translation are induced and persistent cellular proliferation ensues, mediated in part by β-catenin/T-cell factor-dependent transcriptional activation. Cellular proliferation is stimulated by as little as 5 x 10-¹⁰ mol/L BFT. Conclusions: To our knowledge, BFT is the first bacterial toxin reported to activate T-cell factordependent β-catenin nuclear signaling in intestinal epithelial cells. These results suggest that genetic evolution of this common colonic commensal has rendered an organism with the potential to contribute to oncogenic transformation in the colon.