Background free imaging of single mRNAs in live cells using split fluorescent proteins

Bin Wu; Jiahao Chen; Robert H. Singer

doi:10.1038/srep03615

Background free imaging of single mRNAs in live cells using split fluorescent proteins

Bin Wu, Jiahao Chen, Robert H. Singer

Research output: Contribution to journal › Article › peer-review

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Abstract

We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

Original language	English (US)
Article number	3615
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep03615
State	Published - Jan 9 2014
Externally published	Yes

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title = "Background free imaging of single mRNAs in live cells using split fluorescent proteins",

abstract = "We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.",

author = "Bin Wu and Jiahao Chen and Singer, {Robert H.}",

note = "Funding Information: This work was supported by the grant GM84364 and EB 013571 from National Institutes of Health to R.H.S. The plasmid eqFP670 was a kind gift from Dr. Vladislav Verkusha, Albert Einstein College of Medicine. The authors thank Xiuhua Meng and Jefferey A. Chao for cloning.",

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doi = "10.1038/srep03615",

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AU - Wu, Bin

AU - Chen, Jiahao

AU - Singer, Robert H.

N1 - Funding Information: This work was supported by the grant GM84364 and EB 013571 from National Institutes of Health to R.H.S. The plasmid eqFP670 was a kind gift from Dr. Vladislav Verkusha, Albert Einstein College of Medicine. The authors thank Xiuhua Meng and Jefferey A. Chao for cloning.

PY - 2014/1/9

Y1 - 2014/1/9

N2 - We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

AB - We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

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Background free imaging of single mRNAs in live cells using split fluorescent proteins

Abstract

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