Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)

Ted M. Dawson; R. Tyler McCabe; Suzanne S. Stensaas; James K. Wamsley

doi:10.1111/j.1471-4159.1987.tb00962.x

Autoradiographic Evidence of [³H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)

Ted M. Dawson, R. Tyler McCabe, Suzanne S. Stensaas, James K. Wamsley

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Abstract: The distribution of dopamine D‐1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D‐l receptors were localized by using [³H]SCH 23390 as a ligand. Our results indicated that [³H]‐SCH 23390 binding to slide‐mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D‐l receptors, with a B_max value of 11.2 × 1.3 fmol/mg tissue. The K_D values for [³H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [³H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D‐l receptor.

Original language	English (US)
Pages (from-to)	789-796
Number of pages	8
Journal	Journal of Neurochemistry
Volume	49
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-4159.1987.tb00962.x
State	Published - Sep 1987
Externally published	Yes

Keywords

Autoradiography
Brodmann's area 9
Dopamine D‐l receptor
Human brain
Prefrontal cortex
[H]SCH 23390

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1111/j.1471-4159.1987.tb00962.x

Cite this

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title = "Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)",

abstract = "Abstract: The distribution of dopamine D‐1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D‐l receptors were localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]‐SCH 23390 binding to slide‐mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D‐l receptors, with a Bmax value of 11.2 × 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D‐l receptor.",

keywords = "Autoradiography, Brodmann's area 9, Dopamine D‐l receptor, Human brain, Prefrontal cortex, [H]SCH 23390",

author = "Dawson, {Ted M.} and McCabe, {R. Tyler} and Stensaas, {Suzanne S.} and Wamsley, {James K.}",

year = "1987",

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TY - JOUR

T1 - Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)

AU - Dawson, Ted M.

AU - McCabe, R. Tyler

AU - Stensaas, Suzanne S.

AU - Wamsley, James K.

PY - 1987/9

Y1 - 1987/9

N2 - Abstract: The distribution of dopamine D‐1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D‐l receptors were localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]‐SCH 23390 binding to slide‐mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D‐l receptors, with a Bmax value of 11.2 × 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D‐l receptor.

AB - Abstract: The distribution of dopamine D‐1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D‐l receptors were localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]‐SCH 23390 binding to slide‐mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D‐l receptors, with a Bmax value of 11.2 × 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D‐l receptor.

KW - Autoradiography

KW - Brodmann's area 9

KW - Dopamine D‐l receptor

KW - Human brain

KW - Prefrontal cortex

KW - [H]SCH 23390

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