Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA

J. I. Cohen, B. Rosenblum, S. M. Feinstone, J. Ticehurst, R. H. Purcell

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


RNA transcripts of hepatitis A virus (HAV) HM-175 cDNA from atenuated, cell culture-adapted HAV were infectious in cell culture. A full-length HAV cDNA from wild-type HAV (propagated in marmosets in vivo) was constructed. Chimeric cDNAs that contained portions of both wild-type and attenuated genomes were produced. Oligonucleotide-directed mutagenesis was used to engineer a point mutation into the VP1 gene of attenuated HAV cDNA, so that the sequence of this capsid protein would be identical to that of the wild-type virus. Transfection of monkey kidney cells with RNA transcripts from several of the chimeric cDNAs and from the mutagenized cDNA induced production of HAV. Comparison of the growth of aatenuated, wild-type, chimeric, and mutant viruses in vitro indicated that teh P2-P3 (nonstructural protein) region is important for cell culture adaptation of the virus; the 5' noncoding region may also contribute to adaptation, but to a lesser extent. Inoculation of marmosets with transfection-derived virus also suggested that the P2-P3 region plays an important role in attenuation of HAV HM-175.

Original languageEnglish (US)
Pages (from-to)5364-5370
Number of pages7
JournalJournal of virology
Issue number12
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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