Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides.

M. M. Greenberg

Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides.

M. M. Greenberg

Research output: Contribution to journal › Article › peer-review

Abstract

Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid-phase oligonucleotide synthesis supports. However, 3-oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.

Original language	English (US)
Pages (from-to)	Unit 4.5
Journal	Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
Volume	Chapter 4
State	Published - May 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

Cite this

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title = "Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides.",

abstract = "Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid-phase oligonucleotide synthesis supports. However, 3-oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.",

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AB - Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid-phase oligonucleotide synthesis supports. However, 3-oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.

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AN - SCOPUS:43049161030

SN - 1934-9270

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JO - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]

JF - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]

ER -

Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides.

Abstract

ASJC Scopus subject areas

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