TY - JOUR
T1 - Asymmetric AB3 Miktoarm Star Polymers
T2 - Synthesis, Self-Assembly, and Study of Micelle Stability Using AF4 for Efficient Drug Delivery
AU - Moquin, Alexandre
AU - Sharma, Anjali
AU - Cui, Yiming
AU - Lau, Anthony
AU - Maysinger, Dusica
AU - Kakkar, Ashok
N1 - Funding Information:
We would like to thank Prof. F.M. Winnik for the access to the AF4 instrument and other facilities. The work was supported in part by a grant of the Canadian Institutes of Health Research (CIHR, MOP-119425) awarded to DM. AM would like to thank the Canadian Institutes for Health Research for the postdoctoral financial support. AK would like to thank Natural Sciences and Engineering Research Council (NSERC, Canada), Fonds de Recherche du Qu ebec-Nature et technologies (FRQNT, Quebec, Canada), and Center for Self-assembled Chemical Structures (FQRNT, Quebec, Canada) for financial support.
Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - A simple and versatile methodology, which employs a combination of ring-opening polymerization and alkyne-azide click chemistry to synthesize amphiphilic AB3 miktoarm stars, is reported. Their aqueous self-assembly behavior was studied using dynamic light scattering, fluorescence, and asymmetrical flow field-flow fractionation (AF4). AB3 miktoarm stars form micelles which incorporate curcumin with high efficiency, and significantly reduce the viability of glioblastoma cells in spheroids. We demonstrate that AF4 is an effective technique to determine the size distribution of self-assembled structures exposed to a biological medium. The ease, with which asymmetric AB3 miktoarm polymers are constructed, provides a platform that can be widely employed to deliver a variety of lipophilic drugs. AB3 (PCL-PEG3) miktoarm polymers form micelles and incorporate large amount of hydrophobic drug (curcumin). Size distribution of the micelles is narrow as determined by AF4 fractionation. Curcumin-loaded AB3 micelles exert significant cell loss in glioblastoma (GBM) spheroids.
AB - A simple and versatile methodology, which employs a combination of ring-opening polymerization and alkyne-azide click chemistry to synthesize amphiphilic AB3 miktoarm stars, is reported. Their aqueous self-assembly behavior was studied using dynamic light scattering, fluorescence, and asymmetrical flow field-flow fractionation (AF4). AB3 miktoarm stars form micelles which incorporate curcumin with high efficiency, and significantly reduce the viability of glioblastoma cells in spheroids. We demonstrate that AF4 is an effective technique to determine the size distribution of self-assembled structures exposed to a biological medium. The ease, with which asymmetric AB3 miktoarm polymers are constructed, provides a platform that can be widely employed to deliver a variety of lipophilic drugs. AB3 (PCL-PEG3) miktoarm polymers form micelles and incorporate large amount of hydrophobic drug (curcumin). Size distribution of the micelles is narrow as determined by AF4 fractionation. Curcumin-loaded AB3 micelles exert significant cell loss in glioblastoma (GBM) spheroids.
KW - amphiphilic miktoarm star polymer
KW - drug delivery systems
KW - field-flow fractionation
KW - glioblastoma spheroids
KW - micelles
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U2 - 10.1002/mabi.201500186
DO - 10.1002/mabi.201500186
M3 - Article
C2 - 26259625
AN - SCOPUS:84955215549
SN - 1616-5187
VL - 15
SP - 1744
EP - 1754
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 12
ER -