Astroglial Calcium Signaling Encodes Sleep Need in Drosophila

Ian D. Blum, Mehmet F. Keleş, El Sayed Baz, Emily Han, Kristen Park, Skylar Luu, Habon Issa, Matt Brown, Margaret C.W. Ho, Masashi Tabuchi, Sha Liu, Mark N. Wu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Sleep is under homeostatic control, whereby increasing wakefulness generates sleep need and triggers sleep drive. However, the molecular and cellular pathways by which sleep need is encoded are poorly understood. In addition, the mechanisms underlying both how and when sleep need is transformed to sleep drive are unknown. Here, using ex vivo and in vivo imaging, we show in Drosophila that astroglial Ca2+ signaling increases with sleep need. We demonstrate that this signaling is dependent on a specific L-type Ca2+ channel and is necessary for homeostatic sleep rebound. Thermogenetically increasing Ca2+ in astrocytes induces persistent sleep behavior, and we exploit this phenotype to conduct a genetic screen for genes required for the homeostatic regulation of sleep. From this large-scale screen, we identify TyrRII, a monoaminergic receptor required in astrocytes for sleep homeostasis. TyrRII levels rise following sleep deprivation in a Ca2+-dependent manner, promoting further increases in astrocytic Ca2+ and resulting in a positive-feedback loop. Moreover, our findings suggest that astrocytes then transmit this sleep need to a sleep drive circuit by upregulating and releasing the interleukin-1 analog Spätzle, which then acts on Toll receptors on R5 neurons. These findings define astroglial Ca2+ signaling mechanisms encoding sleep need and reveal dynamic properties of the sleep homeostatic control system.

Original languageEnglish (US)
Pages (from-to)150-162.e7
JournalCurrent Biology
Issue number1
StatePublished - Jan 11 2021


  • Drosophila
  • astrocyte
  • astroglial
  • calcium
  • homeostasis
  • homeostatic
  • sleep

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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