TY - JOUR
T1 - Association between Anti-Cd4 antibodies and a decline in Cd4+lymphocytes in human immunodeficiency virus type 1 seroconverters
AU - Multicenter AIDS Cohort Study
AU - Keay, Susan
AU - Wecksler, Wayne
AU - Wasserman, Steven S.
AU - Margolick, Joseph
AU - Farzadegan, Homayoon
AU - Saah, Alfred
AU - Graham, Neil
AU - Munoz, Alvaro
AU - Carey, Vincent
AU - Hoover, Donald
AU - Jacobson, Lisa
AU - Su, Sol
AU - Galai, Noya
AU - Kirby, Jill
AU - Phair, John
AU - Chmiel, John
AU - Sheridan, K.
AU - Cohen, Bruce
AU - Wolinsky, Steven
AU - Detels, Roger
AU - Vischer, Barbara
AU - Fahey, John
AU - Giorgi, Janis
AU - Dudley, Jan
AU - Taylor, Jeremy
AU - Lee, Moon
AU - Rinaldo, Charles
AU - Ho, Monto
AU - Kingsley, Lawrence
AU - Gupta, Phalguni
AU - Winkelstein, A.
AU - Schrager, Lewis
AU - Vermund, Sten
AU - Kaslow, Richard
AU - Van Raden, Mark
AU - Seminara, Daniela
N1 - Funding Information:
Received 12 April 1994; revised I August 1994. Presented in part: 93rd general meeting ofthe American Society for Microbiology, Atlanta, May 1993 (abstract T-22). All samples were obtained through procedures approved by the Human Volunteers Research Committee at the University of Maryland or Johns Hopkins University. No author has a commercial or other association that might pose a conflict of interest for the material presented. Grant support: Infectious Diseases Society of America (Burroughs Wellcome Young Investigator Award in Virology to S.K.): National Institutes of Health (AI-32535 and RR-00722). Reprints or correspondence: Dr. Susan Keay, Research Service, VA Medical Center, Room 38-184,10 N. Greene St., Baltimore, MD 21201. • Members are listed after the text.
PY - 1995/2
Y1 - 1995/2
N2 - Serum specimens (n = 161) from 31 persons before and after human immunodeficiency virus type 1 (HIV-1) seroconversion were tested for anti-CD4 antibodies, These antibodies were detected by both ELISA and Western blot in 55% (17/31) of subjects when HIV-1 seroconversion was detected and in 26% (8/31) from sera obtained 6-24 months earlier, A decrease in CD4+cell number was associated more with development of anti-CD4 antibodies or peak anti-CD4 antibody activity than with development of anti-HIV-1 antibodies. Quantitative DNA polymerase chain reaction assay of peripheral blood mononuclear cells from 7 seroconverters showed evidence of HIV-1 infection in 4 of 4 specimens obtained after HIV-1 seroconversion but was nonreactive for 12 of 12 specimens obtained before HIV-1 seroconversion, including 4 specimens positive for anti-CD4 antibodies by ELISA and Western blot. Therefore, anti-CD4 antibodies are frequently present in the sera of HIV-I-infected persons before and at the time HIV-1 seroconversion is detectable and are associated with a decline in CD4+cell counts, but they are not a marker for HIV-1 infection in seronegative persons.
AB - Serum specimens (n = 161) from 31 persons before and after human immunodeficiency virus type 1 (HIV-1) seroconversion were tested for anti-CD4 antibodies, These antibodies were detected by both ELISA and Western blot in 55% (17/31) of subjects when HIV-1 seroconversion was detected and in 26% (8/31) from sera obtained 6-24 months earlier, A decrease in CD4+cell number was associated more with development of anti-CD4 antibodies or peak anti-CD4 antibody activity than with development of anti-HIV-1 antibodies. Quantitative DNA polymerase chain reaction assay of peripheral blood mononuclear cells from 7 seroconverters showed evidence of HIV-1 infection in 4 of 4 specimens obtained after HIV-1 seroconversion but was nonreactive for 12 of 12 specimens obtained before HIV-1 seroconversion, including 4 specimens positive for anti-CD4 antibodies by ELISA and Western blot. Therefore, anti-CD4 antibodies are frequently present in the sera of HIV-I-infected persons before and at the time HIV-1 seroconversion is detectable and are associated with a decline in CD4+cell counts, but they are not a marker for HIV-1 infection in seronegative persons.
UR - http://www.scopus.com/inward/record.url?scp=0028831108&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028831108&partnerID=8YFLogxK
U2 - 10.1093/infdis/171.2.312
DO - 10.1093/infdis/171.2.312
M3 - Article
C2 - 7844366
AN - SCOPUS:0028831108
SN - 0022-1899
VL - 171
SP - 312
EP - 319
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -