Assignment of human preprothyrotropin-releasing hormone (TRH) gene to chromosome 3

Masanobu Yamada; Fredric E. Wondisford; Sally Radovick; Yuko Nakayama; Bruce D. Weintraub; John F. Wilber

doi:10.1007/BF01233209

Assignment of human preprothyrotropin-releasing hormone (TRH) gene to chromosome 3

Masanobu Yamada, Fredric E. Wondisford, Sally Radovick, Yuko Nakayama, Bruce D. Weintraub, John F. Wilber

School of Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The human gene encoding preproTRH (thyrotropin-releasing hormone) was assigned to chromosome 3, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a³²P-labeled human preproTRH cDNA labeled by the method of random priming (3). Hybridization of the cDNA probe to a human specific 4.8-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human preproTRH gene. No hybridization, by contrast, was seen with human preproTRH cDNA probe and hamster DNA after EcoRI treatment. Results from 29 somatic cell hybrids corroborated unequivocally that the human preproTRH gene can be assigned to human chromosome 3.

Original language	English (US)
Pages (from-to)	97-100
Number of pages	4
Journal	Somatic Cell and Molecular Genetics
Volume	17
Issue number	1
DOIs	https://doi.org/10.1007/BF01233209
State	Published - Jan 1 1991

ASJC Scopus subject areas

Genetics
Cell Biology

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10.1007/BF01233209

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title = "Assignment of human preprothyrotropin-releasing hormone (TRH) gene to chromosome 3",

abstract = "The human gene encoding preproTRH (thyrotropin-releasing hormone) was assigned to chromosome 3, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a32P-labeled human preproTRH cDNA labeled by the method of random priming (3). Hybridization of the cDNA probe to a human specific 4.8-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human preproTRH gene. No hybridization, by contrast, was seen with human preproTRH cDNA probe and hamster DNA after EcoRI treatment. Results from 29 somatic cell hybrids corroborated unequivocally that the human preproTRH gene can be assigned to human chromosome 3.",

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AU - Yamada, Masanobu

AU - Wondisford, Fredric E.

AU - Radovick, Sally

AU - Nakayama, Yuko

AU - Weintraub, Bruce D.

AU - Wilber, John F.

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AB - The human gene encoding preproTRH (thyrotropin-releasing hormone) was assigned to chromosome 3, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a32P-labeled human preproTRH cDNA labeled by the method of random priming (3). Hybridization of the cDNA probe to a human specific 4.8-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human preproTRH gene. No hybridization, by contrast, was seen with human preproTRH cDNA probe and hamster DNA after EcoRI treatment. Results from 29 somatic cell hybrids corroborated unequivocally that the human preproTRH gene can be assigned to human chromosome 3.

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Assignment of human preprothyrotropin-releasing hormone (TRH) gene to chromosome 3

Abstract

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