Assessing spatiotemporal and functional organization of mitochondrial networks

Felix T. Kurz, Miguel A. Aon, Brian O’Rourke, Antonis A. Armoundas

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations


The functional and spatiotemporal organization of mitochondrial redox signaling networks can be studied in detail in cardiac myocytes and neurons by assessing the time-resolved signaling traits of their individual mitochondrial components. Perturbations of the mitochondrial network through oxidative stress can lead to coordinated, cluster-bound behavior in the form of synchronized limit-cycle oscillations of mitochondrial inner membrane potentials. These oscillations are facilitated by both structural coupling through changes in the local redox balance and signaling microdomains and functional coupling that is yet poorly understood. Thus, quantifiable measures of both coupling mechanisms, local dynamic mitochondrial coupling constants and functional clustering coefficients, are likely to offer valuable information on mitochondrial network organization. We provide step-by-step methodologies on how to acquire and assess these measures for inner membrane potential fluorescence fluctuations in laser-scanning two-photon microscope recordings of cardiac myocytes and neurons, that can be applied to other tissues as well.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages20
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cardiac myocyte
  • Mitochondrial clustering
  • Mitochondrial coupling
  • Mitochondrial network
  • Mitochondrial oscillator
  • Wavelets

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Assessing spatiotemporal and functional organization of mitochondrial networks'. Together they form a unique fingerprint.

Cite this