Abstract
Functional AMPA receptors (AMPARs) are tetrameric complexes formed by four identical (homomeric) or similar (heteromeric) subunits. Variations in the number and composition of AMPARs on the plasma membrane impact synaptic strength, neurodevelopment, and brain disorders. While the mechanisms mediating oligomerization of AMPARs are not well understood, they form the template for defining the number and preferential assembly of AMPARs. In this chapter we describe the application of two methods, blue-native PAGE (BN-PAGE) and fluorescence-detection size-exclusion chromatography (FSEC), to delineate the oligomeric state of AMPAR complexes and factors that determine the oligomerization process.
Original language | English (US) |
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Title of host publication | Ionotropic Glutamate Receptor Technologies |
Publisher | Springer New York |
Pages | 3-14 |
Number of pages | 12 |
ISBN (Electronic) | 9781493928125 |
ISBN (Print) | 9781493928118 |
DOIs | |
State | Published - Sep 25 2015 |
Externally published | Yes |
Keywords
- Blue-native PAGE
- Fluorescence-detection size-exclusion chromatography
- Ionotropic glutamate receptors
- M4 segment
- Transmembrane domain
ASJC Scopus subject areas
- General Medicine
- General Neuroscience