Artificial reporter gene providing MRI contrast based on proton exchange

Assaf Gilad; Michael T. McMahon; Piotr Walczak; Paul T. Winnard; Venu Raman; Hanneke W.M. Van Laarhoven; Cynthia M. Skoglund; Jeff W.M. Bulte; Peter C.M. Van Zijl

doi:10.1038/nbt1277

Artificial reporter gene providing MRI contrast based on proton exchange

Assaf Gilad, Michael T. McMahon, Piotr Walczak, Paul T. Winnard, Venu Raman, Hanneke W.M. Van Laarhoven, Cynthia M. Skoglund, Jeff W.M. Bulte, Peter C.M. Van Zijl

Research output: Contribution to journal › Article › peer-review

306 Scopus citations

Abstract

Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

Original language	English (US)
Pages (from-to)	217-219
Number of pages	3
Journal	Nature biotechnology
Volume	25
Issue number	2
DOIs	https://doi.org/10.1038/nbt1277
State	Published - Feb 2007

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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title = "Artificial reporter gene providing MRI contrast based on proton exchange",

abstract = "Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.",

author = "Assaf Gilad and McMahon, {Michael T.} and Piotr Walczak and Winnard, {Paul T.} and Venu Raman and {Van Laarhoven}, {Hanneke W.M.} and Skoglund, {Cynthia M.} and Bulte, {Jeff W.M.} and {Van Zijl}, {Peter C.M.}",

note = "Funding Information: We thank D.A. Kedziorek, V.P. Chacko and J. Zhang for experimental assistance. Supported by National Institutes of Health grants CA103175, NS045062, EB005252 and K01 EB006394.",

year = "2007",

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doi = "10.1038/nbt1277",

language = "English (US)",

volume = "25",

pages = "217--219",

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T1 - Artificial reporter gene providing MRI contrast based on proton exchange

AU - Gilad, Assaf

AU - McMahon, Michael T.

AU - Walczak, Piotr

AU - Winnard, Paul T.

AU - Raman, Venu

AU - Van Laarhoven, Hanneke W.M.

AU - Skoglund, Cynthia M.

AU - Bulte, Jeff W.M.

AU - Van Zijl, Peter C.M.

N1 - Funding Information: We thank D.A. Kedziorek, V.P. Chacko and J. Zhang for experimental assistance. Supported by National Institutes of Health grants CA103175, NS045062, EB005252 and K01 EB006394.

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N2 - Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

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Artificial reporter gene providing MRI contrast based on proton exchange

Abstract

ASJC Scopus subject areas

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