Arg347Cys polymorphism of α1A-adrenoceptor gene is associated with blood pressure response to nifedipine GITS in Chinese hypertensive patients

Our objectives were to evaluate whether polymorphisms in the α1A- and Β2-adrenoceptor genes influence blood pressure response to nifedipine gastrointestinal therapeutic system (GITS). Hypertensive patients received daily treatment with an oral dosage of 30 mg nifedipine GITS for 16 days. Genotypes of the Arg347Cys polymorphism in the α1A-adrenoceptor gene and the Arg16Gly and Gln27Glu polymorphisms in the Β2-adrenoceptor gene were determined by TaqMan single-nucleotide polymorphism genotyping assay. The sixteenth-day steady-state plasma concentration of nifedipine was measured using HPLC with UV detection. Multivariate linear regression was performed in a total of 447 patients to evaluate the effects of these polymorphisms on blood pressure response to nifedipine GITS. Patients carrying the Cys347 allele of the α1A-adrenoceptor gene had a greater systolic blood pressure reduction than did those carrying two Arg347 alleles of the α1A-adrenoceptor gene (32.514.0 versus 27.315.5 mm Hg, respectively, P0.006). However, diastolic blood pressure reduction was not associated with the Arg347Cys polymorphism in the α1A-adrenoceptor gene. In addition, no significant associations were observed between blood pressure reduction and two polymorphisms in the Β2-adrenoceptor gene. Our data suggest that the Arg347Cys polymorphism in the α1A-adrenoceptor gene may be used to predict blood pressure response to nifedipine GITS in Chinese hypertensive patients.