Applications of Functional Protein Microarrays in Basic and Clinical Research

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations

Abstract

The protein microarray technology provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput manner. It is viewed as a new tool that overcomes the limitation of DNA microarrays. On the basis of its application, protein microarrays fall into two major classes: analytical and functional protein microarrays. In addition, tissue or cell lysates can also be directly spotted on a slide to form the so-called " reverse-phase" protein microarray. In the last decade, applications of functional protein microarrays in particular have flourished in studying protein function and construction of networks and pathways. In this chapter, we will review the recent advancements in the protein microarray technology, followed by presenting a series of examples to illustrate the power and versatility of protein microarrays in both basic and clinical research. As a powerful technology platform, it would not be surprising if protein microarrays will become one of the leading technologies in proteomic and diagnostic fields in the next decade.

Original languageEnglish (US)
Title of host publicationAdvances in Genetics
PublisherAcademic Press Inc.
Pages123-155
Number of pages33
DOIs
StatePublished - 2012

Publication series

NameAdvances in Genetics
Volume79
ISSN (Print)0065-2660

Keywords

  • Antibody microarray
  • Biomarkers
  • Networks
  • Protein microarray
  • Protein posttranslational modification
  • Reverse-phase protein microarray
  • Serum profiling

ASJC Scopus subject areas

  • Genetics

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