Application of RLEP real-time PCR for detection of m. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy

Wen Yan; Yan Xing; Lian Chao Yuan; Rong De Yang; Fu Yue Tan; Ying Zhang; Huan Ying Li

doi:10.4269/ajtmh.13-0659

Application of RLEP real-time PCR for detection of m. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy

Wen Yan, Yan Xing, Lian Chao Yuan, Rong De Yang, Fu Yue Tan, Ying Zhang, Huan Ying Li

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.

Original language	English (US)
Pages (from-to)	524-529
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	90
Issue number	3
DOIs	https://doi.org/10.4269/ajtmh.13-0659
State	Published - Mar 2014
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Virology
Infectious Diseases

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title = "Application of RLEP real-time PCR for detection of m. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy",

abstract = "The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.",

author = "Wen Yan and Yan Xing and Yuan, {Lian Chao} and {De Yang}, Rong and {Yue Tan}, Fu and Ying Zhang and Li, {Huan Ying}",

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AU - Yan, Wen

AU - Xing, Yan

AU - Yuan, Lian Chao

AU - De Yang, Rong

AU - Yue Tan, Fu

AU - Zhang, Ying

AU - Li, Huan Ying

PY - 2014/3

Y1 - 2014/3

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Application of RLEP real-time PCR for detection of m. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy

Abstract

ASJC Scopus subject areas

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