Antisecretory mechanisms of Peptide YY in rat distal colon

Edward E. Whang; Oscar J. Hines; Joseph R. Reeve; Daniel Grandt; James A. Moser; Anton J. Bilchik; Michael J. Zinner; David W. McFadden; Stanley W. Ashley

doi:10.1023/A:1018869116284

Antisecretory mechanisms of Peptide YY in rat distal colon

Edward E. Whang, Oscar J. Hines, Joseph R. Reeve, Daniel Grandt, James A. Moser, Anton J. Bilchik, Michael J. Zinner, David W. McFadden, Stanley W. Ashley

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro³⁴)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro³⁴)-PYY and PYY-(3-36).

Original language	English (US)
Pages (from-to)	1121-1127
Number of pages	7
Journal	Digestive diseases and sciences
Volume	42
Issue number	6
DOIs	https://doi.org/10.1023/A:1018869116284
State	Published - 1997
Externally published	Yes

Keywords

Colon
Intestinal secretion
Neuropeptide Y
Peptide YY

ASJC Scopus subject areas

Physiology
Gastroenterology

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10.1023/A:1018869116284

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@article{dd53847d1a794a8b9c916f5481b03283,

title = "Antisecretory mechanisms of Peptide YY in rat distal colon",

abstract = "Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).",

keywords = "Colon, Intestinal secretion, Neuropeptide Y, Peptide YY",

author = "Whang, {Edward E.} and Hines, {Oscar J.} and Reeve, {Joseph R.} and Daniel Grandt and Moser, {James A.} and Bilchik, {Anton J.} and Zinner, {Michael J.} and McFadden, {David W.} and Ashley, {Stanley W.}",

note = "Funding Information: Manuscript received August 23, 1996; revised manuscript received January 30, 1997; accepted February 9, 1997. From the Departments of Surgery and Medicine, UCLA School of Medicine, Los Angeles, California; and Department of Surgery, Brigham and Women{\textquoteright} s Hospital, Boston, Massachusetts. This work was supported by National Institute of Health grants R29-DK-4732 6 (S.W.A.) and R01-DK-3987 0 (M.J.Z.). Address for reprint requests: Dr. Stanley Ashley, Brigham Surgical Group, 110 Cypress St., Brookline, Massachusetts 02146.",

year = "1997",

doi = "10.1023/A:1018869116284",

language = "English (US)",

volume = "42",

pages = "1121--1127",

journal = "Digestive diseases and sciences",

issn = "0163-2116",

publisher = "Plenum Publishers",

number = "6",

}

TY - JOUR

T1 - Antisecretory mechanisms of Peptide YY in rat distal colon

AU - Whang, Edward E.

AU - Hines, Oscar J.

AU - Reeve, Joseph R.

AU - Grandt, Daniel

AU - Moser, James A.

AU - Bilchik, Anton J.

AU - Zinner, Michael J.

AU - McFadden, David W.

AU - Ashley, Stanley W.

N1 - Funding Information: Manuscript received August 23, 1996; revised manuscript received January 30, 1997; accepted February 9, 1997. From the Departments of Surgery and Medicine, UCLA School of Medicine, Los Angeles, California; and Department of Surgery, Brigham and Women’ s Hospital, Boston, Massachusetts. This work was supported by National Institute of Health grants R29-DK-4732 6 (S.W.A.) and R01-DK-3987 0 (M.J.Z.). Address for reprint requests: Dr. Stanley Ashley, Brigham Surgical Group, 110 Cypress St., Brookline, Massachusetts 02146.

PY - 1997

Y1 - 1997

N2 - Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).

AB - Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).

KW - Colon

KW - Intestinal secretion

KW - Neuropeptide Y

KW - Peptide YY

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DO - 10.1023/A:1018869116284

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C2 - 9201071

AN - SCOPUS:0030807207

SN - 0163-2116

VL - 42

SP - 1121

EP - 1127

JO - Digestive diseases and sciences

JF - Digestive diseases and sciences

IS - 6

ER -

Antisecretory mechanisms of Peptide YY in rat distal colon

Abstract

Keywords

ASJC Scopus subject areas

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