Antiproliferation effects of immunoconjugate of monoclonal anticollage IV-genistein on human lens epithelial cells in vitro

Fang Liu, Wei Kong, Jun Kong, Jin Song Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

• Aim: To study the antiproliferation effects of immunoconjugate of monoclonal anticollagenIV-genistein on human lens epithelial cell (LEC) in vitro. • Methods: The immunological activities of the antibody in immunoconjugate were determined by immunohistochemistry method. The morphological changes of LEC cells treated by immunoconjugate were observed by inverted microscopy and fluorescence microscopy after Acridine orange staining. The effect of immunoconjugate on the proliferation of hLEC cells was examined by tetrazolium salt (MTT) assay method. • Results: The activity of antibody maintained well in the immunoconjugate. The shape of LEC cells changed differently after being treated with immunoconjugate in different time. Apoptotic bodies could be found under fluorescence microscopy after LEC cells treated with immunoconjugate for 48 hours with AO staining. Immunoconjugate effectively inhibited LEC cells growth in a time-dependent manner in MTT assay. The antiproliferation effect of immunoconjugate was more sensitive compared to genistein with statistical significance. • Conclusion: The immunoconjugate of monoclonal anti-collageIV-genistein conjungated by active ester intermediate can effectively inhibit LEC cells from proliferation.

Original languageEnglish (US)
Pages (from-to)651-653
Number of pages3
JournalInternational Journal of Ophthalmology
Volume7
Issue number3
StatePublished - Jun 2007
Externally publishedYes

Keywords

  • Anti-collagen IV monoclonal antibody
  • Epithelial cells
  • Genistein
  • Immunoconjugate
  • Lens

ASJC Scopus subject areas

  • Ophthalmology

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