Antioxidant activity of carotenoid lutein in vitro and in vivo

Edakkadath R. Sindhu, Korengath C. Preethi, Ramadasan Kuttan

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Carotenoid lutein was evaluated for its antioxidant potential both in vitro and in vivo. Lutein was found to scavenge superoxide radicals, hydroxyl radicals and inhibited in vitro lipid peroxidation. Concentrations needed for 50 % inhibition (IC50) were 21, 1.75 and 2.2 μg/mL respectively. It scavenged 2,2-diphenyl-1-picryl hydrazyl (IC50 35 μg/mL) and nitric oxide radicals (IC50 3.8 μg/mL) while 2,2-azobis-3-ethylbenzthiozoline-6-sulfonic acid radicals were inhibited at higher concentration. Ferric reducing power (50%) of lutein was found to be equal 0.3μmols/mL of FeSO4.7H2O. Its oral administration inhibited superoxide generation in macrophages in vivo by 34.18, 64.32 and 70.22 % at doses of 50, 100 and 250 mg/kg body weight. The oral administration of lutein in mice for 1 month significantly increased the activity of catalase, superoxide dismutase, glutathione reductase and glutathione in blood and liver while the activity of glutathione peroxidase and glutathione-S-transferase were found to be increased in the liver tissue. Implication of these results in terms of its role in reducing degenerative diseases is discussed.

Original languageEnglish (US)
Pages (from-to)843-848
Number of pages6
JournalIndian journal of experimental biology
Issue number8
StatePublished - Aug 1 2010
Externally publishedYes


  • Antioxidants
  • Carotenoid
  • Free radicals
  • Lutein
  • Macrophages
  • Macular pigment

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology


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