Abstract
The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-Ia cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.
Original language | English (US) |
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Pages (from-to) | 157-165 |
Number of pages | 9 |
Journal | Journal of Immunology |
Volume | 133 |
Issue number | 1 |
State | Published - 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology