@inbook{2cc13f3dde3643a9a0e23c66a77265ce,
title = "Antibody specificity profiling using protein microarrays",
abstract = "Antibodies are the most widely used reagent for isolation and detection of specific proteins. However, using antibodies that are not highly specific in these studies can generate inaccurate and misleading data. Protein microarrays offer a platform by which antibody cross-reactivity against a broad range of cellular antigens can be simultaneously and quantitatively profiled. This protocol describes in detail the process of array pretreatment, antibody binding, washing, scanning and quantitative analysis of antibody specificity.",
keywords = "Antibody specificity, Cross-reactivity, Monoclonal antibodies, Protein microarray",
author = "Pedro Ramos-L{\'o}pez and Jos{\'e} Irizarry and Ignacio Pino and Seth Blackshaw",
note = "Funding Information: This work was supported by NIH grant U54HG006434. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7841-0_14",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "223--229",
booktitle = "Methods in Molecular Biology",
}