Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude

Godfree Mlambo; Susan L. Mutambu; Takafira Mduluza; White Soko; Joel Mbedzi; James Chivenga; David E. Lanar; Sanjay Singh; Daniel Carucci; Armin Gemperli; Nirbhay Kumar

doi:10.1016/j.actatropica.2006.09.012

Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude

Godfree Mlambo, Susan L. Mutambu, Takafira Mduluza, White Soko, Joel Mbedzi, James Chivenga, David E. Lanar, Sanjay Singh, Daniel Carucci, Armin Gemperli, Nirbhay Kumar

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Antibody levels against malaria antigens were measured among patients presenting with uncomplicated malaria at health centers from three locations in Zimbabwe (Bindura, Chiredzi and Kariba) that are distinct with regard to altitude and climatic conditions. Antibody levels were determined by ELISA using the antigens, apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), circumsporozoite surface protein (CSP), merozoite surface protein 1 (MSP-1) and Pfg27. For all the antigens tested, IgG and IgM levels were higher for Bindura (altitude 1100 m) compared to Kariba (<600 m, altitude) and Chiredzi (∼600 m, altitude) with the exception of IgG and IgM to AMA-1 and EBA-175 which were similar between Chiredzi and Bindura. Plasma samples were further analyzed for their functional activity by testing their ability to inhibit the growth of Plasmodium falciparum in culture. Our results, determined by microscopy and verified by the LDH assay revealed that plasma from the three locations had similar inhibitory activity against the growth of P. falciparum in vitro. Our data revealed that highest growth inhibition correlated with the highest levels of MSP-1 antibody values.

Original language	English (US)
Pages (from-to)	70-78
Number of pages	9
Journal	Acta Tropica
Volume	100
Issue number	1-2
DOIs	https://doi.org/10.1016/j.actatropica.2006.09.012
State	Published - Nov 2006

Keywords

Antibody responses
Malaria vaccine
P. falciparum
Transmission
Zimbabwe

ASJC Scopus subject areas

Parasitology
veterinary (miscalleneous)
Insect Science
Infectious Diseases

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10.1016/j.actatropica.2006.09.012

Cite this

@article{3d58ab0127db4b0289b7d0b2e90127e0,

title = "Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude",

abstract = "Antibody levels against malaria antigens were measured among patients presenting with uncomplicated malaria at health centers from three locations in Zimbabwe (Bindura, Chiredzi and Kariba) that are distinct with regard to altitude and climatic conditions. Antibody levels were determined by ELISA using the antigens, apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), circumsporozoite surface protein (CSP), merozoite surface protein 1 (MSP-1) and Pfg27. For all the antigens tested, IgG and IgM levels were higher for Bindura (altitude 1100 m) compared to Kariba (<600 m, altitude) and Chiredzi (∼600 m, altitude) with the exception of IgG and IgM to AMA-1 and EBA-175 which were similar between Chiredzi and Bindura. Plasma samples were further analyzed for their functional activity by testing their ability to inhibit the growth of Plasmodium falciparum in culture. Our results, determined by microscopy and verified by the LDH assay revealed that plasma from the three locations had similar inhibitory activity against the growth of P. falciparum in vitro. Our data revealed that highest growth inhibition correlated with the highest levels of MSP-1 antibody values.",

keywords = "Antibody responses, Malaria vaccine, P. falciparum, Transmission, Zimbabwe",

author = "Godfree Mlambo and Mutambu, {Susan L.} and Takafira Mduluza and White Soko and Joel Mbedzi and James Chivenga and Lanar, {David E.} and Sanjay Singh and Daniel Carucci and Armin Gemperli and Nirbhay Kumar",

note = "Funding Information: We would like to thank the National Institute of Health Research in Zimbabwe for providing samples that were used for this study. We thank Fogarty International Center Training Grant (TW001587) for funding this work. Research in the NK laboratory is supported by NIH grants and the supply of human RBC is supported by NIH grant RR00052.",

year = "2006",

month = nov,

doi = "10.1016/j.actatropica.2006.09.012",

language = "English (US)",

volume = "100",

pages = "70--78",

journal = "Acta Tropica",

issn = "0001-706X",

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number = "1-2",

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TY - JOUR

T1 - Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude

AU - Mlambo, Godfree

AU - Mutambu, Susan L.

AU - Mduluza, Takafira

AU - Soko, White

AU - Mbedzi, Joel

AU - Chivenga, James

AU - Lanar, David E.

AU - Singh, Sanjay

AU - Carucci, Daniel

AU - Gemperli, Armin

AU - Kumar, Nirbhay

N1 - Funding Information: We would like to thank the National Institute of Health Research in Zimbabwe for providing samples that were used for this study. We thank Fogarty International Center Training Grant (TW001587) for funding this work. Research in the NK laboratory is supported by NIH grants and the supply of human RBC is supported by NIH grant RR00052.

PY - 2006/11

Y1 - 2006/11

N2 - Antibody levels against malaria antigens were measured among patients presenting with uncomplicated malaria at health centers from three locations in Zimbabwe (Bindura, Chiredzi and Kariba) that are distinct with regard to altitude and climatic conditions. Antibody levels were determined by ELISA using the antigens, apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), circumsporozoite surface protein (CSP), merozoite surface protein 1 (MSP-1) and Pfg27. For all the antigens tested, IgG and IgM levels were higher for Bindura (altitude 1100 m) compared to Kariba (<600 m, altitude) and Chiredzi (∼600 m, altitude) with the exception of IgG and IgM to AMA-1 and EBA-175 which were similar between Chiredzi and Bindura. Plasma samples were further analyzed for their functional activity by testing their ability to inhibit the growth of Plasmodium falciparum in culture. Our results, determined by microscopy and verified by the LDH assay revealed that plasma from the three locations had similar inhibitory activity against the growth of P. falciparum in vitro. Our data revealed that highest growth inhibition correlated with the highest levels of MSP-1 antibody values.

AB - Antibody levels against malaria antigens were measured among patients presenting with uncomplicated malaria at health centers from three locations in Zimbabwe (Bindura, Chiredzi and Kariba) that are distinct with regard to altitude and climatic conditions. Antibody levels were determined by ELISA using the antigens, apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), circumsporozoite surface protein (CSP), merozoite surface protein 1 (MSP-1) and Pfg27. For all the antigens tested, IgG and IgM levels were higher for Bindura (altitude 1100 m) compared to Kariba (<600 m, altitude) and Chiredzi (∼600 m, altitude) with the exception of IgG and IgM to AMA-1 and EBA-175 which were similar between Chiredzi and Bindura. Plasma samples were further analyzed for their functional activity by testing their ability to inhibit the growth of Plasmodium falciparum in culture. Our results, determined by microscopy and verified by the LDH assay revealed that plasma from the three locations had similar inhibitory activity against the growth of P. falciparum in vitro. Our data revealed that highest growth inhibition correlated with the highest levels of MSP-1 antibody values.

KW - Antibody responses

KW - Malaria vaccine

KW - P. falciparum

KW - Transmission

KW - Zimbabwe

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ER -

Antibody responses to Plasmodium falciparum vaccine candidate antigens in three areas distinct with respect to altitude

Abstract

Keywords

ASJC Scopus subject areas

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