Antibody-antigen binding in organic solvents

Alan J. Russell, Laura J. Trudel, Paul L. Skipper, John D. Groopman, Steven R. Tannenbaum, Alexander M. Klibanov

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Jan 16 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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