TY - JOUR
T1 - Antibodies to ICAM-1 protect kidneys in severe ischemic reperfusion injury
AU - Rabb, Hamid
AU - Mendiola, Carmelo C.
AU - Saba, Sabiha R.
AU - Dietz, John R.
AU - Wayne Smith, C.
AU - Bonventre, Joseph V.
AU - Ramirez, German
PY - 1995/6/6
Y1 - 1995/6/6
N2 - ICAM-1 has been implicated in the pathophysiology of ischemic-reperfusion injury in a number of organs, but its role in mediating severe ischemic-reperfusion injury in the kidney has not been extensively studied. Uninephrectomized Sprague Dawley rats were pretreated with either control monoclonal antibody (mAb) or mAb to ICAM-1 and subjected to 60 min of renal artery occlusion. The serum creatinine, complete blood count and kidney histo-pathological damage scores (PDS) (Scale:0-4) were assessed prior to and 24 hours after ischemia. Mean serum creatinine (mg/dl) 24 hours after ischemia was significantly decreased in the anti-ICAM-1 group (1.38 ± 0.23, p<0.001) compared to control (2.87 ± 0.34). PDS was also reduced in anti-ICAM-1 (2.55 ± 0.20, p<0.05) group compared to control (3.35 ± 0.30). These data demonstrate that blocking ICAM-1 significantly mitigates severe ischemic acute renal failure, findings which may lead to improved therapy for this condition.
AB - ICAM-1 has been implicated in the pathophysiology of ischemic-reperfusion injury in a number of organs, but its role in mediating severe ischemic-reperfusion injury in the kidney has not been extensively studied. Uninephrectomized Sprague Dawley rats were pretreated with either control monoclonal antibody (mAb) or mAb to ICAM-1 and subjected to 60 min of renal artery occlusion. The serum creatinine, complete blood count and kidney histo-pathological damage scores (PDS) (Scale:0-4) were assessed prior to and 24 hours after ischemia. Mean serum creatinine (mg/dl) 24 hours after ischemia was significantly decreased in the anti-ICAM-1 group (1.38 ± 0.23, p<0.001) compared to control (2.87 ± 0.34). PDS was also reduced in anti-ICAM-1 (2.55 ± 0.20, p<0.05) group compared to control (3.35 ± 0.30). These data demonstrate that blocking ICAM-1 significantly mitigates severe ischemic acute renal failure, findings which may lead to improved therapy for this condition.
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U2 - 10.1006/bbrc.1995.1779
DO - 10.1006/bbrc.1995.1779
M3 - Article
C2 - 7779111
AN - SCOPUS:0029073219
SN - 0006-291X
VL - 211
SP - 67
EP - 73
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -