TY - JOUR
T1 - Anti-inflammatory effects of sacral nerve stimulation
T2 - A novel spinal afferent and vagal efferent pathway
AU - Tu, Lei
AU - Gharibani, Payam
AU - Zhang, Nina
AU - Yin, Jieyun
AU - Chen, Jiande
N1 - Funding Information:
This work was supported by the DARPA BTO ElectRx program under the auspices of Dr. Doug Weber, Contract No. N66001-15-2-4059 (J. Chen) and the Ludwig-Bayless Scientist Award (J. Yin).
Publisher Copyright:
© 2020 the American Physiological Society.
PY - 2020
Y1 - 2020
N2 - Sacral nerve stimulation (SNS) was reported to improve 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats. The aim of this study was to investigate whether the SNS anti-inflammatory effect is mediated via the local sacral splanchnic nerve or the spinal afferent-vagal efferent-colon pathway. Under general anesthesia, rats were administrated with TNBS intrarectally, and bipolar SNS electrodes were implanted unilaterally at S3. The sacral and vagal nerves were severed at different locations for the assessment of the neural pathway. SNS for 10 days improved colonic inflammation only in groups with intact afferent sacral nerve and vagus efferent nerve. SNS markedly increased acetylcholine and anti-inflammatory cytokines (IL-10) and decreased myeloperoxidase and proinflammatory cytokines (IL-2, IL-17A, and TNF-α) in colon tissues. SNS increased the number of c-fos-positive cells in the brain stem and normalized vagal activity measured by spectral analysis of heart rate variability. SNS exerts an anti-inflammatory effect on TNBS-induced colitis by enhancing vagal activity mediated mainly via the spinal afferent-brain stem-vagal efferent-colon pathway.
AB - Sacral nerve stimulation (SNS) was reported to improve 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats. The aim of this study was to investigate whether the SNS anti-inflammatory effect is mediated via the local sacral splanchnic nerve or the spinal afferent-vagal efferent-colon pathway. Under general anesthesia, rats were administrated with TNBS intrarectally, and bipolar SNS electrodes were implanted unilaterally at S3. The sacral and vagal nerves were severed at different locations for the assessment of the neural pathway. SNS for 10 days improved colonic inflammation only in groups with intact afferent sacral nerve and vagus efferent nerve. SNS markedly increased acetylcholine and anti-inflammatory cytokines (IL-10) and decreased myeloperoxidase and proinflammatory cytokines (IL-2, IL-17A, and TNF-α) in colon tissues. SNS increased the number of c-fos-positive cells in the brain stem and normalized vagal activity measured by spectral analysis of heart rate variability. SNS exerts an anti-inflammatory effect on TNBS-induced colitis by enhancing vagal activity mediated mainly via the spinal afferent-brain stem-vagal efferent-colon pathway.
KW - Autonomic function
KW - Inflammatory bowel diseases
KW - Inflammatory cytokines
KW - Neurectomy
KW - Sacral nerve stimulation
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U2 - 10.1152/ajpgi.00330.2019
DO - 10.1152/ajpgi.00330.2019
M3 - Article
C2 - 32068444
AN - SCOPUS:85082146298
SN - 0193-1857
VL - 318
SP - G624-G634
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 4
ER -