TY - JOUR
T1 - Anti-basal ganglia antibody abnormalities in Sydenham chorea
AU - Singer, Harvey S.
AU - Loiselle, Christopher R.
AU - Lee, Olivia
AU - Garvey, Marjorie A.
AU - Grus, Franz H.
N1 - Funding Information:
This work was supported, in part, by grants from the Tourette Syndrome Association and NIH RO1 NS37706. The authors thank Dr. Susan Swedo for her suggestions related to this project and for review of the manuscript.
PY - 2003/3
Y1 - 2003/3
N2 - Anti-basal ganglia antibodies (ABGA) were measured in nine children with Sydenham chorea (SC) and compared to nine controls. Enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) methods were used to detect ABGA against supernatant (S1), pellet, and synaptosomal preparations from adult and pediatric postmortem caudate, putamen, and globus pallidus. ELISA optical density (OD) values were higher in SC patients than controls across all preparations, but did not reach a level of significance. Although WB identified multiple bands in all subjects, discriminant analysis showed that the mean binding patterns of SC patients were significantly different from control, most notably in the caudate S1 fraction (Wilks' λ=0.011, p<0.0001). Numerous antigens contributed to differences between groups; the two most defining molecular masses were at 126 and 113 kDa. In contrast to WB with discriminant analysis, ELISA measurements did not significantly differentiate between the SC group and controls.
AB - Anti-basal ganglia antibodies (ABGA) were measured in nine children with Sydenham chorea (SC) and compared to nine controls. Enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) methods were used to detect ABGA against supernatant (S1), pellet, and synaptosomal preparations from adult and pediatric postmortem caudate, putamen, and globus pallidus. ELISA optical density (OD) values were higher in SC patients than controls across all preparations, but did not reach a level of significance. Although WB identified multiple bands in all subjects, discriminant analysis showed that the mean binding patterns of SC patients were significantly different from control, most notably in the caudate S1 fraction (Wilks' λ=0.011, p<0.0001). Numerous antigens contributed to differences between groups; the two most defining molecular masses were at 126 and 113 kDa. In contrast to WB with discriminant analysis, ELISA measurements did not significantly differentiate between the SC group and controls.
KW - Anti-basal ganglia antibodies
KW - Discriminant analysis
KW - ELISA
KW - Sydenham chorea
KW - Western blot
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U2 - 10.1016/S0165-5728(03)00034-1
DO - 10.1016/S0165-5728(03)00034-1
M3 - Article
C2 - 12620655
AN - SCOPUS:0037337835
SN - 0165-5728
VL - 136
SP - 154
EP - 161
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -