TY - JOUR
T1 - Antagonistic regulation of the gibberellic acid response during stem growth in rice
AU - Nagai, Keisuke
AU - Mori, Yoshinao
AU - Ishikawa, Shin
AU - Furuta, Tomoyuki
AU - Gamuyao, Rico
AU - Niimi, Yoko
AU - Hobo, Tokunori
AU - Fukuda, Moyuri
AU - Kojima, Mikiko
AU - Takebayashi, Yumiko
AU - Fukushima, Atsushi
AU - Himuro, Yasuyo
AU - Kobayashi, Masatomo
AU - Ackley, Wataru
AU - Hisano, Hiroshi
AU - Sato, Kazuhiro
AU - Yoshida, Aya
AU - Wu, Jianzhong
AU - Sakakibara, Hitoshi
AU - Sato, Yutaka
AU - Tsuji, Hiroyuki
AU - Akagi, Takashi
AU - Ashikari, Motoyuki
N1 - Funding Information:
Acknowledgements We thank H. Morishima (deceased 2010) for encouragement to do this work and K. Suzuki, Y. Kondo, M. Koike, A. Minami, S. Reuscher, N. Yamaji, J. F. Ma and S. Sasaki for technical assistance; M. Mikami, S. Toki and M. Endo (NARO) for providing the CRISPR–Cas9 vectors; M. Ueguchi-Tanaka for providing the SLR1 antibody; and R. B. Angeles-Shim for English language editing. Deepwater rice, wild rice accessions and barley cv. Golden Promise were provided by the National Bioresource Project (NBRP) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. This work was supported by the CREST programme of the Japan Science and Technology Agency (JST) (JPMJCR13B1); by the SATREPS programme (JPMJSA1706) of the JST and Japan International Cooperation Agency (JICA); by MEXT/JSPS KAKENHI (16K18565, 16H06466, 17H06473 and 19K15815); and by Genomics for Agricultural Innovation (QTL5003) of the Ministry of Agriculture, Forestry, and Fisheries of Japan (MAFF). This study was supported in part by RIKEN-Nagoya University Science and Technology Hub.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/8/6
Y1 - 2020/8/6
N2 - The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid1–3. Here we show that internode stem elongation in rice is regulated antagonistically by an ‘accelerator’ and a ‘decelerator’ in concert with gibberellic acid. Expression of a gene we name ACCELERATOR OF INTERNODE ELONGATION 1 (ACE1), which encodes a protein of unknown function, confers cells of the intercalary meristematic region with the competence for cell division, leading to internode elongation in the presence of gibberellic acid. By contrast, upregulation of DECELERATOR OF INTERNODE ELONGATION 1 (DEC1), which encodes a zinc-finger transcription factor, suppresses internode elongation, whereas downregulation of DEC1 allows internode elongation. We also show that the mechanism of internode elongation that is mediated by ACE1 and DEC1 is conserved in the Gramineae family. Furthermore, an analysis of genetic diversity suggests that mutations in ACE1 and DEC1 have historically contributed to the selection of shorter plants in domesticated populations of rice to increase their resistance to lodging, and of taller plants in wild species of rice for adaptation to growth in deep water. Our identification of these antagonistic regulatory factors enhances our understanding of the gibberellic acid response as an additional mechanism that regulates internode elongation and environmental fitness, beyond biosynthesis and gibberellic acid signal transduction.
AB - The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid1–3. Here we show that internode stem elongation in rice is regulated antagonistically by an ‘accelerator’ and a ‘decelerator’ in concert with gibberellic acid. Expression of a gene we name ACCELERATOR OF INTERNODE ELONGATION 1 (ACE1), which encodes a protein of unknown function, confers cells of the intercalary meristematic region with the competence for cell division, leading to internode elongation in the presence of gibberellic acid. By contrast, upregulation of DECELERATOR OF INTERNODE ELONGATION 1 (DEC1), which encodes a zinc-finger transcription factor, suppresses internode elongation, whereas downregulation of DEC1 allows internode elongation. We also show that the mechanism of internode elongation that is mediated by ACE1 and DEC1 is conserved in the Gramineae family. Furthermore, an analysis of genetic diversity suggests that mutations in ACE1 and DEC1 have historically contributed to the selection of shorter plants in domesticated populations of rice to increase their resistance to lodging, and of taller plants in wild species of rice for adaptation to growth in deep water. Our identification of these antagonistic regulatory factors enhances our understanding of the gibberellic acid response as an additional mechanism that regulates internode elongation and environmental fitness, beyond biosynthesis and gibberellic acid signal transduction.
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UR - http://www.scopus.com/inward/citedby.url?scp=85087986529&partnerID=8YFLogxK
U2 - 10.1038/s41586-020-2501-8
DO - 10.1038/s41586-020-2501-8
M3 - Article
C2 - 32669710
AN - SCOPUS:85087986529
SN - 0028-0836
VL - 584
SP - 109
EP - 114
JO - Nature
JF - Nature
IS - 7819
ER -