Anopheles gambiae lacking AgTRIO probe inefficiently on a mammalian host

Yu Min Chuang; Yuemei Dong; Helen Stone; Selma Abouneameh; Xu Dong Tang; Hamidah Raduwan; George Dimopoulos; Erol Fikrig

doi:10.1016/j.celrep.2024.114600

Anopheles gambiae lacking AgTRIO probe inefficiently on a mammalian host

Yu Min Chuang, Yuemei Dong, Helen Stone, Selma Abouneameh, Xu Dong Tang, Hamidah Raduwan, George Dimopoulos, Erol Fikrig

Research output: Contribution to journal › Article › peer-review

Abstract

Malaria is initiated as Plasmodium sporozoites are injected into the dermis when an infected mosquito probes on a vertebrate host for a blood meal. Factors in the mosquito saliva, such as AgTRIO, can alter the ability of Anopheles gambiae to transmit Plasmodium. We therefore used CRISPR-Cas9-mediated genome editing to generate AgTRIO knockout (KO) A. gambiae and examined the ability of these mosquitoes to probe on a vertebrate host. AgTRIO KO mosquitoes showed a diminished host probing capacity and required repetitive probing to locate a blood resource to complete a blood meal. This increased probing resulted in enhanced Plasmodium transmission to the vertebrate host. Our data demonstrate the importance of the A. gambiae saliva protein AgTRIO in probing and its influence on the ability of mosquitoes to transmit malaria.

Original language	English (US)
Article number	114600
Journal	Cell Reports
Volume	43
Issue number	8
DOIs	https://doi.org/10.1016/j.celrep.2024.114600
State	Published - Aug 27 2024
Externally published	Yes

Keywords

AgTRIO
CP: Metabolism
Plasmodium transmission
blood meal
mosquito
saliva protein

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/j.celrep.2024.114600

Cite this

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title = "Anopheles gambiae lacking AgTRIO probe inefficiently on a mammalian host",

abstract = "Malaria is initiated as Plasmodium sporozoites are injected into the dermis when an infected mosquito probes on a vertebrate host for a blood meal. Factors in the mosquito saliva, such as AgTRIO, can alter the ability of Anopheles gambiae to transmit Plasmodium. We therefore used CRISPR-Cas9-mediated genome editing to generate AgTRIO knockout (KO) A. gambiae and examined the ability of these mosquitoes to probe on a vertebrate host. AgTRIO KO mosquitoes showed a diminished host probing capacity and required repetitive probing to locate a blood resource to complete a blood meal. This increased probing resulted in enhanced Plasmodium transmission to the vertebrate host. Our data demonstrate the importance of the A. gambiae saliva protein AgTRIO in probing and its influence on the ability of mosquitoes to transmit malaria.",

keywords = "AgTRIO, CP: Metabolism, Plasmodium transmission, blood meal, mosquito, saliva protein",

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doi = "10.1016/j.celrep.2024.114600",

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AU - Chuang, Yu Min

AU - Dong, Yuemei

AU - Stone, Helen

AU - Abouneameh, Selma

AU - Tang, Xu Dong

AU - Raduwan, Hamidah

AU - Dimopoulos, George

AU - Fikrig, Erol

PY - 2024/8/27

Y1 - 2024/8/27

N2 - Malaria is initiated as Plasmodium sporozoites are injected into the dermis when an infected mosquito probes on a vertebrate host for a blood meal. Factors in the mosquito saliva, such as AgTRIO, can alter the ability of Anopheles gambiae to transmit Plasmodium. We therefore used CRISPR-Cas9-mediated genome editing to generate AgTRIO knockout (KO) A. gambiae and examined the ability of these mosquitoes to probe on a vertebrate host. AgTRIO KO mosquitoes showed a diminished host probing capacity and required repetitive probing to locate a blood resource to complete a blood meal. This increased probing resulted in enhanced Plasmodium transmission to the vertebrate host. Our data demonstrate the importance of the A. gambiae saliva protein AgTRIO in probing and its influence on the ability of mosquitoes to transmit malaria.

AB - Malaria is initiated as Plasmodium sporozoites are injected into the dermis when an infected mosquito probes on a vertebrate host for a blood meal. Factors in the mosquito saliva, such as AgTRIO, can alter the ability of Anopheles gambiae to transmit Plasmodium. We therefore used CRISPR-Cas9-mediated genome editing to generate AgTRIO knockout (KO) A. gambiae and examined the ability of these mosquitoes to probe on a vertebrate host. AgTRIO KO mosquitoes showed a diminished host probing capacity and required repetitive probing to locate a blood resource to complete a blood meal. This increased probing resulted in enhanced Plasmodium transmission to the vertebrate host. Our data demonstrate the importance of the A. gambiae saliva protein AgTRIO in probing and its influence on the ability of mosquitoes to transmit malaria.

KW - AgTRIO

KW - CP: Metabolism

KW - Plasmodium transmission

KW - blood meal

KW - mosquito

KW - saliva protein

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JO - Cell Reports

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ER -

Anopheles gambiae lacking AgTRIO probe inefficiently on a mammalian host

Abstract

Keywords

ASJC Scopus subject areas

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