Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

Amina S. Woods; Katrin Fuhrer; Marc Gonin; Tom Egan; Michael Ugarov; Kent J. Gillig; J. Albert Schultz

Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

Amina S. Woods, Katrin Fuhrer, Marc Gonin, Tom Egan, Michael Ugarov, Kent J. Gillig, J. Albert Schultz

Research output: Contribution to journal › Article › peer-review

Abstract

Matrix-assisted laser desorption ionization-ion mobility- orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is a new technique that allows laser desorbed ions to be preseparated on the basis of their shape prior to mass analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angiotensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore, the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Journal of Biomolecular Techniques
Volume	14
Issue number	1
State	Published - Mar 2003
Externally published	Yes

Keywords

Acetylcholine
Angiotensin
Ion mobility
Phosphorylated pep-tides

ASJC Scopus subject areas

Molecular Biology

Cite this

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abstract = "Matrix-assisted laser desorption ionization-ion mobility- orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is a new technique that allows laser desorbed ions to be preseparated on the basis of their shape prior to mass analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angiotensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore, the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.",

keywords = "Acetylcholine, Angiotensin, Ion mobility, Phosphorylated pep-tides",

author = "Woods, {Amina S.} and Katrin Fuhrer and Marc Gonin and Tom Egan and Michael Ugarov and Gillig, {Kent J.} and {Albert Schultz}, J.",

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language = "English (US)",

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T1 - Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

AU - Woods, Amina S.

AU - Fuhrer, Katrin

AU - Gonin, Marc

AU - Egan, Tom

AU - Ugarov, Michael

AU - Gillig, Kent J.

AU - Albert Schultz, J.

PY - 2003/3

Y1 - 2003/3

N2 - Matrix-assisted laser desorption ionization-ion mobility- orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is a new technique that allows laser desorbed ions to be preseparated on the basis of their shape prior to mass analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angiotensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore, the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.

AB - Matrix-assisted laser desorption ionization-ion mobility- orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is a new technique that allows laser desorbed ions to be preseparated on the basis of their shape prior to mass analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angiotensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore, the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.

KW - Acetylcholine

KW - Angiotensin

KW - Ion mobility

KW - Phosphorylated pep-tides

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Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

Abstract

Keywords

ASJC Scopus subject areas

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