TY - JOUR
T1 - Analytical methods for assessing exposure to 4-aminobiphenyl based on protein adduct formation
AU - Skipper, Paul L.
AU - Bryant, Matthew S.
AU - Tannenbaum, Steven R.
AU - Groopman, John D.
PY - 1986/8
Y1 - 1986/8
N2 - Past studies with animals have demonstrated that 4-aminobiphenyl (ABP) administration results in the formation of appreciable amounts of adducts between the carcinogen and both serum albumin and hemoglobin. The hemoglobin adduct is relatively stable in vivo, but may be readily hydrolyzed in vitro to regenerate ABP. The formation of this adduct reflects a mixed-function oxidase-mediated metabolic pathway operating directly on the amine. The predominant albumin adduct, 3-(tryptophan-N1-y1)-4-acetylaminobiphenyl, reflects the contribution of N-acetyltransferase activity as weii as mixed function oxidase activity to the overall metabolism. The simultaneous measurement of these two different adducts thus offers an opportunity to investigate the role of both ABP and acetylator phenotype in bladder carcinogenesis. An analytical method, using gas chromatography coupled with electron capture detection, was developed to quantitate ABP adducted to hemoglobin.
AB - Past studies with animals have demonstrated that 4-aminobiphenyl (ABP) administration results in the formation of appreciable amounts of adducts between the carcinogen and both serum albumin and hemoglobin. The hemoglobin adduct is relatively stable in vivo, but may be readily hydrolyzed in vitro to regenerate ABP. The formation of this adduct reflects a mixed-function oxidase-mediated metabolic pathway operating directly on the amine. The predominant albumin adduct, 3-(tryptophan-N1-y1)-4-acetylaminobiphenyl, reflects the contribution of N-acetyltransferase activity as weii as mixed function oxidase activity to the overall metabolism. The simultaneous measurement of these two different adducts thus offers an opportunity to investigate the role of both ABP and acetylator phenotype in bladder carcinogenesis. An analytical method, using gas chromatography coupled with electron capture detection, was developed to quantitate ABP adducted to hemoglobin.
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U2 - 10.1097/00043764-198608000-00021
DO - 10.1097/00043764-198608000-00021
M3 - Article
C2 - 3746485
AN - SCOPUS:0022444902
SN - 1076-2752
VL - 28
SP - 643
EP - 646
JO - Journal of Occupational and Environmental Medicine
JF - Journal of Occupational and Environmental Medicine
IS - 8
ER -