Abstract
In vitro studies of BrdU-dependent sister chromatid differential staining typically employ two cycles of BrdU incorporation. Experiments are described which determined the actual fraction of both S-phases that the rat embryonic fibroblasts (Rat-1) cells had to traverse in order to show distinctive differential staining. Following synchronization of cells by a combination of serum deprivation and hydroxyurea blockage, sister chromatid differential staining, labelling index, mitotic index, and per cent DNA replication are determined. Results indicate that only ~50% of the first S-phase is necessary in order to show distinctive differential staining. The importance of this finding to studies of cellular proliferation using BrdU incorporation is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 527-532 |
| Number of pages | 6 |
| Journal | Cell and Tissue Kinetics |
| Volume | 19 |
| Issue number | 5 |
| State | Published - 1986 |
| Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology