TY - JOUR
T1 - Analysis of protein phosphorylation using mass spectrometry
T2 - Deciphering the phosphoproteome
AU - Mann, Matthias
AU - Ong, Shao En
AU - Grønborg, Mads
AU - Steen, Hanno
AU - Jensen, Ole N.
AU - Pandey, Akhilesh
PY - 2002/6/1
Y1 - 2002/6/1
N2 - In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.
AB - In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.
UR - http://www.scopus.com/inward/record.url?scp=0036605185&partnerID=8YFLogxK
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U2 - 10.1016/S0167-7799(02)01944-3
DO - 10.1016/S0167-7799(02)01944-3
M3 - Article
C2 - 12007495
AN - SCOPUS:0036605185
SN - 0167-7799
VL - 20
SP - 261
EP - 268
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 6
ER -