Abstract
Paired serum, urine, and finger-prick whole blood dried on filter paper were analyzed by immunoradiometric assay (IRMA) for filarial antigen using Brugia malayi-specific rabbit antibody. Nine sera and 6 urines from the 10 paired serum-urine samples obtained from individuals with microfilaremia contained IRMA detectable filarial antigen. In contrast, all serum and urine specimens from patients with chronic infections, endemic and non-endemic controls were negative. Whole blood eluted from filter paper spots contained IRMA detectable material; their degree of positivity agreed well with IRMA binding levels obtained with paired urines. Reduced recovery of antigen dried on filter paper was observed at antigen levels <10 ng/ml equivalents, presumably due to irreversible absorption onto the filter paper. Urine and finger-prick filter paper blood specimens can be used in the diagnosis of microfilaremic infections that have been associated with circulating antigen in the blood.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 351-365 |
| Number of pages | 15 |
| Journal | Journal of Immunoassay |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 1987 |
| Externally published | Yes |
Keywords
- Filariasis
- diagnosis
- filarial antigens
- filter paper blood
- immunoradiometric assay
- urine
ASJC Scopus subject areas
- Immunology
- Pharmacology