@article{4621d5dfac1f47e9ab4b45329b5f8e93,
title = "Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade",
abstract = "Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this {"}AstroPath{"} whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163+PD-L1- myeloid cells and CD8+FoxP3+PD-1low/mid T cells. These features were combined to stratify long-term survival after anti-PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.",
author = "Sneha Berry and Giraldo, {Nicolas A.} and Green, {Benjamin F.} and Cottrell, {Tricia R.} and Stein, {Julie E.} and Engle, {Elizabeth L.} and Haiying Xu and Aleksandra Ogurtsova and Charles Roberts and Daphne Wang and Peter Nguyen and Qingfeng Zhu and Sigfredo Soto-Diaz and Jose Loyola and Sander, {Inbal B.} and Wong, {Pok Fai} and Shlomit Jessel and Joshua Doyle and Danielle Signer and Richard Wilton and Roskes, {Jeffrey S.} and Margaret Eminizer and Seyoun Park and Sunshine, {Joel C.} and Jaffee, {Elizabeth M.} and Alexander Baras and {De Marzo}, {Angelo M.} and Topalian, {Suzanne L.} and Harriet Kluger and Leslie Cope and Lipson, {Evan J.} and Ludmila Danilova and Anders, {Robert A.} and Rimm, {David L.} and Pardoll, {Drew M.} and Szalay, {Alexander S.} and Taube, {Janis M.}",
note = "Publisher Copyright: {\textcopyright} 2021 American Association for the Advancement of Science. All rights reserved.",
year = "2021",
month = jun,
day = "11",
doi = "10.1126/science.aba2609",
language = "English (US)",
volume = "372",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6547",
}