@inbook{891a8be19661447ab4d744e8a7ecacc2,
title = "Analysis of Hypoxia-Inducible Factor 1α Expression and its Effects on Invasion and Metastasis",
abstract = "Hypoxia-inducible factor 1 (HIF-1) plays an important role in human cancer cell invasion and metastasis. As a result, overexpression of the HIF-1α subunit in biopsy specimens is associated with increased patient mortality in several common cancers, including breast adenocarcinoma and oropharyngeal squamous cell carcinoma. Here, we describe methods for immunohistochemical detection of HIF-1α in tumor biopsy sections and ex vivo assays for analyzing the effects of hypoxia and HIF-1 on cancer cell invasiveness and cell-cell adhesion.",
author = "Balaji Krishnamachary and Semenza, {Gregg L.}",
year = "2007",
doi = "10.1016/S0076-6879(07)35017-9",
language = "English (US)",
isbn = "9780123739704",
series = "Methods in Enzymology",
pages = "347--354",
editor = "Helmut Sies and Bernhard Brune",
booktitle = "Oxygen Biology and Hypoxia",
}