Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India

Sivaram Gunisetty, Kaustuv Nayak, Ramesh Chandra Rai, Y. Chawla, Elluri Seetharami Reddy, C. Aggarwal, Deepti Maheshwari, Harekrushna Panda, Nasim Akhtar Ansari, Prabhat Singh, Manpreet Kaur, Kritika Dixit, Pragati Sharma, P. Bhatnagar, Lalita Priyamvada, Siddhartha Kumar Bhaumik, Syed Fazil Ahamed, Rosario Vivek, Pratima Ray, A. ShetPoonam Coshic, Rakesh Lodha, Sushil Kumar Kabra, Dil Afroze, A. Yousuf, Rafi Ahmed, Kaja Murali-Krishna, A. Chandele

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

Original languageEnglish (US)
Pages (from-to)S57-S63
JournalInternational Journal of Infectious Diseases
StatePublished - Jul 2019


  • Dengue
  • India
  • Memory B-cell
  • Neutralizing antibody

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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