TY - JOUR
T1 - Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India
AU - Gunisetty, Sivaram
AU - Nayak, Kaustuv
AU - Chandra Rai, Ramesh
AU - Chawla, Y.
AU - Reddy, Elluri Seetharami
AU - Aggarwal, C.
AU - Maheshwari, Deepti
AU - Panda, Harekrushna
AU - Ansari, Nasim Akhtar
AU - Singh, Prabhat
AU - Kaur, Manpreet
AU - Dixit, Kritika
AU - Sharma, Pragati
AU - Bhatnagar, P.
AU - Priyamvada, Lalita
AU - Bhaumik, Siddhartha Kumar
AU - Ahamed, Syed Fazil
AU - Vivek, Rosario
AU - Ray, Pratima
AU - Shet, A.
AU - Coshic, Poonam
AU - Lodha, Rakesh
AU - Kabra, Sushil Kumar
AU - Afroze, Dil
AU - Yousuf, A.
AU - Ahmed, Rafi
AU - Murali-Krishna, Kaja
AU - Chandele, A.
N1 - Funding Information:
This work was supported by National Institutes of Health grants ICIDR 1UO1A/115654 (R. Ahmed). Indo-US Vaccine action program BT/MB. Indo US/VAP/06/2013 (K Murali Krishna). DBT - BT/PR8470/med/29/726/203 (A. Shet).
Funding Information:
This work was supported by National Institutes of Health grants ICIDR 1UO1A/115654 (R. Ahmed). Indo-US Vaccine action program BT/MB. Indo US/VAP/06/2013 (K Murali Krishna). DBT - BT/PR8470/med/29/726/203 (A. Shet). This article is part of a supplement entitled ?Dengue Fever in India? which is sponsored by the Department of Biotechnology, Government of India, and collated by the Translational Health Science and Technology Institute. The authors declare no conflict of interest and funding agency has no role in the designing of the experiments.
Publisher Copyright:
© 2019
PY - 2019/7
Y1 - 2019/7
N2 - Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.
AB - Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.
KW - Dengue
KW - India
KW - Memory B-cell
KW - Neutralizing antibody
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U2 - 10.1016/j.ijid.2019.01.018
DO - 10.1016/j.ijid.2019.01.018
M3 - Article
C2 - 30658170
AN - SCOPUS:85061057738
SN - 1201-9712
VL - 84
SP - S57-S63
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -