Abstract
Retinal ganglion cell (RGC) transplantation has the potential to restore vision in optic neuropathy, but donor neuron survival and retinal integration remain challenging. Here, we present a protocol for ex vivo human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies. The protocol includes microscopy-based analyses to evaluate donor cell engraftment and can be adapted to various donor cell types or culture systems. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021a, 2021b).
Original language | English (US) |
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Article number | 101328 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 2 |
DOIs | |
State | Published - Jun 17 2022 |
Keywords
- Cell Biology
- Cell culture
- Microscopy
- Neuroscience
- Stem Cells
- Tissue Engineering
ASJC Scopus subject areas
- General Medicine
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
- General Neuroscience