Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture

Kevin Y. Zhang; Thomas V. Johnson

doi:10.1016/j.xpro.2022.101328

Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture

Kevin Y. Zhang, Thomas V. Johnson

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Retinal ganglion cell (RGC) transplantation has the potential to restore vision in optic neuropathy, but donor neuron survival and retinal integration remain challenging. Here, we present a protocol for ex vivo human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies. The protocol includes microscopy-based analyses to evaluate donor cell engraftment and can be adapted to various donor cell types or culture systems. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021a, 2021b).

Original language	English (US)
Article number	101328
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101328
State	Published - Jun 17 2022

Keywords

Cell Biology
Cell culture
Microscopy
Neuroscience
Stem Cells
Tissue Engineering

ASJC Scopus subject areas

General Medicine
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

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10.1016/j.xpro.2022.101328

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abstract = "Retinal ganglion cell (RGC) transplantation has the potential to restore vision in optic neuropathy, but donor neuron survival and retinal integration remain challenging. Here, we present a protocol for ex vivo human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies. The protocol includes microscopy-based analyses to evaluate donor cell engraftment and can be adapted to various donor cell types or culture systems. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021a, 2021b).",

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AU - Johnson, Thomas V.

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AB - Retinal ganglion cell (RGC) transplantation has the potential to restore vision in optic neuropathy, but donor neuron survival and retinal integration remain challenging. Here, we present a protocol for ex vivo human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies. The protocol includes microscopy-based analyses to evaluate donor cell engraftment and can be adapted to various donor cell types or culture systems. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021a, 2021b).

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KW - Stem Cells

KW - Tissue Engineering

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Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture

Abstract

Keywords

ASJC Scopus subject areas

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