An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics

Nan Li; Galit Pelled; Assaf A. Gilad; Piotr Walczak; Nitish V. Thakor

doi:10.1109/NER.2011.5910492

An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics

Nan Li, Galit Pelled, Assaf A. Gilad, Piotr Walczak, Nitish V. Thakor

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

An integrated optical system combining optogenetics and laser speckle contrast imaging (LSCI) method was explored. For the first time, LSCI was demonstrated capable of revealing the modulated neuronal behavior induced by light activated halorhodopsin a light sensitive chloride pump. Decreased amplitude and extent of both cerebral blood flow response and oxy-hemoglobin concentration response were revealed when halorhodopsin was activated during the electrical forepaw stimulation. The combination of fast transient neuronal manipulation technique-optogenetics with the high spatio-temporal resolution, wide field of view and minimum invasive imaging method LSCI, brings unique insights into studies on basic brain studies, neurovascular coupling and neurological diseases.

Original language	English (US)
Title of host publication	2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011
Pages	76-79
Number of pages	4
DOIs	https://doi.org/10.1109/NER.2011.5910492
State	Published - Jul 20 2011
Event	2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011 - Cancun, Mexico Duration: Apr 27 2011 → May 1 2011

Publication series

Name	2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011

Other

Other	2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011
Country/Territory	Mexico
City	Cancun
Period	4/27/11 → 5/1/11

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1109/NER.2011.5910492

Cite this

Li, N., Pelled, G., Gilad, A. A., Walczak, P., & Thakor, N. V. (2011). An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics. In 2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011 (pp. 76-79). Article 5910492 (2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011). https://doi.org/10.1109/NER.2011.5910492

An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics. / Li, Nan; Pelled, Galit; Gilad, Assaf A. et al.
2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011. 2011. p. 76-79 5910492 (2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Li, N, Pelled, G, Gilad, AA, Walczak, P & Thakor, NV 2011, An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics. in 2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011., 5910492, 2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011, pp. 76-79, 2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011, Cancun, Mexico, 4/27/11. https://doi.org/10.1109/NER.2011.5910492

Li N, Pelled G, Gilad AA, Walczak P, Thakor NV. An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics. In 2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011. 2011. p. 76-79. 5910492. (2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011). doi: 10.1109/NER.2011.5910492

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abstract = "An integrated optical system combining optogenetics and laser speckle contrast imaging (LSCI) method was explored. For the first time, LSCI was demonstrated capable of revealing the modulated neuronal behavior induced by light activated halorhodopsin a light sensitive chloride pump. Decreased amplitude and extent of both cerebral blood flow response and oxy-hemoglobin concentration response were revealed when halorhodopsin was activated during the electrical forepaw stimulation. The combination of fast transient neuronal manipulation technique-optogenetics with the high spatio-temporal resolution, wide field of view and minimum invasive imaging method LSCI, brings unique insights into studies on basic brain studies, neurovascular coupling and neurological diseases.",

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An in vivo optical system: Control and monitor cortical activity with improved laser speckle contrast imaging and optogenetics

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